Browsing by Author "Devi, Silvera"
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Item ANALISIS AKTIVITAS ENZIM AMILASE PRODUKSI JAMUR TERMOFILIK Aspergillus sp. LBKURCC304 DENGAN SUMBER KARBON BERBEDA(perpustakaan UR, 2021-08) Sitompul, Lorena Omega; Saryono, Saryono; Devi, SilveraThermophilic amylase is great demand in industrial processes and biotechnology. The production of amylase can be affected by carbon sources. In this study, the production of amylase was carried out by Aspergillus sp. LBKURCC304 with several natural carbon sources (cassava, corn, taro, purple sweet potato, potato, breadfruit, canna, gembili, gadung, and sago). Production was carried out in submerged fermentation pH 7 at 50°C for 11 days with an agitation speed of 150 rpm. Determination of amylase activity was using the Nelson-Somogyi method, absorbance was measured using a UVVis spectrophotometer with a wavelength of 540 nm. The results showed that the highest activity was obtained in presence of sago carbohydrates (0.0391 ± 0.0017 U/mL) as a carbon sourceItem ANALISIS INHIBISI DARI INFUSA DAUN DOLAR RAMBAT (Ficus pumila) DAN DAUN JAMBU BIJI (Psidium guajava) TERHADAP AKTIVITAS α-AMILASE(2016-10-12) Fresga, Roy; Dahliaty, Andi; Devi, Silveraα-Amylase is an enzyme that serves as a catalyst of starch hydrolysis to produce maltose in the digestive system. Inhibition of this enzyme can reduce blood glucose levels, especially in type 2 diabetic patients. Thus, the objective of this study were to determine inhibition of aqueous extract (infusa) from climbing fig (Ficus pumila) and guava leaves (Psidium guajava) in fresh and dried condition to α-amylase activities. Inhibition of the samples to α-amylase was determined by Nelson-Somogyi method. The absorbance was measured using spectrophotometry UV-Vis at 540 nm. Acarbose was used as positive control. The results showed that samples were potential inhibitors for α-amylase, i.e., infusa of climbing fig dried (91.91 + 2.43) and infusa of guava dried leaves (84.68 + 5.80). Percent inhibition of these plants were not significantly different from acarbose 0.5% (92.96 ± 0.18).Item ANALISIS PRODUKSI ENZIM AMILASE DARI JAMUR TERMOFILIK Aspergillus sp. LBKURCC304 STRAIN LOKAL BUKIK GADANG SUMATERA BARAT(2020-10) Fadhila, Widylia Fitri; Saryono, Saryono; Devi, SilveraAmylase is an enzyme that hydrolyzes of starch into reducing sugars and can be produced from the fermentation process by several microorganisms, including the thermophilic fungus of Aspergillus sp. LBKURCC304. Thermophilic fungi have an advantage in production of amylase enzyme because they can live at high temperature, so the metabolites such as enzymes and proteins that resulted will be more resistant to high temperature. Based on previous studies, Aspergillus sp. LBKURCC304 has been isolated and produced amylase enzyme qualitatively at 50oC, but quantitative enzyme production has never been done. The purpose of this study was to production of amylase enzyme quantitatively from this isolate, like the optimum activity from amylase crude extract and protein content as well as the optimum amylase specific activity produced every day for 18 days of the fermentation process at 50oC. Nelson-Somogyi and Lowry method were used to determine the enzyme activity and the protein content, respectively. Specific activity was obtained by dividing the amylase enzyme activity with the protein content. The results obtained were analyzed using the Anova and Duncan test. Result for the highest enzyme activity was obtained on the 11th day at 0,0302±0,0041 U/mL, optimum protein content was obtained on the 7th day at 2,2821±0,0632 mg/mL, while the highest specific activity was produced on the 11th day, with 0,0214±0,0005 U/mg. One unit of amylase enzyme activity is defined as the amount of enzyme needed to produce 1 μmol of reducing sugar per minute at 50oC with pH 7.Item ANALISIS TIGA METODE PENENTUAN AKTIVITAS ENZIM α-AMILASE(2021-03) Az Zahra, Elmerrillia Sylvia Haning; Devi, SilveraThe enzyme activity as a biocatalyst can be calculated based on the number of moles of the substrate that is hydrolyzed into product per unit time (Iodine method), or the number of moles of product produced per unit time (2,3-dinitrosalicylic acid (DNS) method and the Nelson-Somogyi (NS) method). This third method are with experimental conditions of pH 7, temperature of 50°C and an incubation time of 30 minutes. The results showed that the α-amylase enzyme activity measured by the DNS method was 0.115 ± 0.002 μmol/minute and the NS method was 0.041 ± 0.025 μmol/minute, while the Iodine method had activity of 2.090 ± 0.005 μmol/minute. The activity data of these three methods after being statistically tested by the Duncan method turned out to be significant between the DNS method (2,3-dinitrosalicylic acid) with the Nelson-Somogyi (NS) method and the Iodine method.Item EFISIENSI ADSORPSI ARANG AKTIF DARI CANGKANG KELAPA SAWIT (Elaeis guineensis Jacq.) SEBAGAI ADSORBEN Escherichia coli DENGAN METODE BATCH(Elfitra, 2023-11) Andini, Jumika; Itnawita, Itnawita; Devi, SilveraPalm shell charcoal (Elaeis guineensis Jacq.) has the potential to be used as an adsorbent to adsorb Escherichia coli (E. coli), however its adsorption efficiency is relatively small. Therefore, to increase its adsorption efficiency in this study palm shell charcoal will be activated with ZnCl2 to adsorb E. coli with the batch method. The aim of this study was to analyze the concentration of ZnCl2 activator variation palm shell on the adsorb E. coli. Palm shell charcoal is made through a carbonization process at a temperature of 400oC for 2 hour, then crushed at size that that passes 50 mesh and is retained in 100 mesh. Charcoal were activated using zinc chloride (ZnCl2) with the concentration of ZnCl2 activator variation 0.5 M, 1 M, 1.5 M, and 2 M. Surface area, pore volume, along pore radius was analyzed using a surface area analyzer using the BET method. Surface morphology using SEM instrument. Based on the results of the BET obtained surface area of 16.1740 m2/g, a pore radius of 2.1763 nm, and a pore volume of 8.8 × 10-3 cc/g. The optimum adsorption conditions with the batch method were obtained at the optimum concentration of ZnCl2 activator of 1.5 M with an average amount of E. coli adsorbed of 5.13 × 108 cells/mL in 1 g of activated carbon or adsorption efficiency of E. coli of 81.95%.Item EFISIENSI ADSORPSI Escherichia coli MENGGUNAKAN ARANG AKTIF CANGKANG KELAPA SAWIT (Elaeis guineensis Jacq.) DENGAN METODE KONTINU(Elfitra, 2023-11) Anggraini, Dewi Sri; Itnawita, Itnawita; Devi, SilveraPalm shells (Elaeis gueneensis Jacq.) is one of the materials that can be used as a good raw material for making active charcoal. Active charcoal can be used as an adsorbent for metals, non-metals and organic compounds. In this research, the potential of active charcoal from palm shell was seen from its ability to adsorb Escherichia coli with the continuous method. This study aims to determine the activator concentration and optimum grain size for adsorption of palm shell activated charcoal against E.coli. The activated palm shell charcoal used is charcoal with grain sizes of 30, 50 and 100 mesh. Surface area, pore volume, and pore radius were analyzed using a surface area analyzer with the BET-BJH method. Palm shell charcoal morphology was analyzed using SEM. Based on the results of the BET obtained surface area of 16.174 m2/g, a pore radius of 2.1763 nm, and a pore volume of 8.8 x 10-3 cc/g. The optimum conditions of adsorption were obtained at the optimum grain size of 50 mesh with an average amount of E.coli adsorbed is 9.17 x 108 cells/mL and the E.coli adsorption percentage is 84.67%.Item Kemampuan Ganoderma Sp Mendegradasi Lignin Pada Beberapa Konsentrasi Lindi Hitam(wahyu sari yeni, 2017-09-13) Martina, Atria; Devi, Silvera; Marlina, SriJamur yang mempunyai aktivitas ligniolitik berpotensi diterapkan pada bidang bioteknologi seperti pada proses biopulping, biobleaching, dekolorisasi dan detoksifikasi senyawa aromatik. Ganoderma sp yang diisolasi dari Taman Nasional Bukit Tigapuluh telah diteliti mempunyai aktivitas ligniolitik. Penelitian ini bertujuan mendeteksi kemampuan Ganoderma sp dalam mendegradasi lignin pada lindi hitam secara fermentasi dibawah permukaan yang diagitasi. Spora Ganoderma sp (11,7 x 103/ml) diinokulasi pada medium N-Limited mengandung lindi hitam dengan konsentrasi 20%, 40% dan 60%. Perlakuan kontrol tanpa diinokulasi Ganoderma sp.Kecepatan agitasi diperlambat pada saat idiofase. Hasil penelitian menunjukkan bahwa inokulasi Ganoderma sp dapat meningkatkan degradasi lignin dan menekan perubahan pH akhir. Degradasi lignin yang terbesar (65,42%) adalah pada konsentrasi lindi 20% dan yang terendah (4,41%) pada tanpa diinokulasi Ganoderma sp dengan konsentrasi lindi hitam 60%. pH akhir medium fermentasi tertinggi sebesar 5,43 diperoleh pada perlakuan tanpa Ganoderma sp. dengan konsentrasi lindi hitam 60% dan terendah sebesar 5,07 ditemukan pada perlakuan dengan Ganoderma sp dan konsentrasi lindi hitam 20%.Item OPTIMALISASI pH PRODUKSI ENZIM AMILASE BAKTERI ENDOFITIK Pseudomonas stutzery LBKURCC46(2016-10-19) Marsiti; Devi, Silvera; SaryonoAmylase is an hydrolase enzyme which hydrolyze starch into maltose and glucose. Amylase has been produced by Pseudomanas stutzery LBKURCC45, LBKURCC46, and LBKURCC55, the endophytic bacteria. Amylase activity was determined based on the reducing sugars formed from starch hydrolisis by amylase with the DNS (dinitrosalicylic acid) method on a UV-Vis spectrophotometer at 540 nm. The highest amylase activity at pH 7.0, 40°C and agitation of 120 rpm was shown by LBKURCC46 bacteria. Furthermore, the optimization of amylase production by LBKURCC46 bacteria was conducted at various pHs (6.0; 6.5; 7.0; 7.5). The results showed that the optimum activity was 27.5308 x 10-3 U/mL at pH 6.5.Item OPTIMALISASI pH PRODUKSI ENZIM AMILASE BAKTERI ENDOFITIK Pseudomonas stutzery LBKURCC53(2016-10-19) Fitriyanti; Devi, Silvera; SaryonoPseudomonas stutzery LBKURCC49, LBKURCC51 and LBKURCC53 are local endofitic bacteria that can produce amylase. Amylase is an enzyme which hydrolyze amilum into maltose and glucose by breaking down the α-1,4 glycosidic linkages. The production of amylase enzyme from microorganisms can be optimized by adjusting the fermentation conditions. The activity of amylase enzyme was determined with the DNS (dinitrosalicylic) methods. The highest amylase enzyme activity at pH 6.0, 40°C and 120 rpm was shown by Pseudomonas stutzery LBKURCC53. Furthermore, optimalization of amylase production by LBKURCC53 was conducted performed at various pHs (6.5; 7.0; 6.0; 7.5). The results showed that the optimum amylase enzyme activity was 66.173 x 10-3 U/mL at pH 6.0Item OPTIMASI PRODUKSI ENZIM AMILASE TERMOFILIK Aspergillus sp. LBKURCC304 DENGAN VARIABEL JENIS SUMBER NITROGEN DAN MINERAL DALAM MEDIA CAIR(Elfitra, 2022-07) Rahmawati, Nur Anis; Devi, Silvera; Saryono, SaryonoThermophilic amylase is an enzyme that is widely used in biotechnology and industry, because it can be used at high temperatures. Nitrogen is an important component that affects the metabolism of microorganisms in producing the enzyme amylase. In this study an attempt was made to optimize the production of the amylase enzyme from the thermophilic fungus Aspergillus sp. LBKURCC304 in submerged fermentation at 50⁰C for 11 days with an agitation speed of 150 rpm. The production media varied the type of nitrogen source (soy flour, tempeh flour and catfish flour) and minerals (FeSO4.7H2O, CaCl2.2H2O, MgSO4.7H2O, MnSO4.H2O and BaCl2.2H2O). The activity of the amylase enzyme produced was determined by the Nelson-Semogyi method, and absorbance was measured at a wavelength of 540 nm using a UV-Vis Spectrophotometer. The results of the study showed that the nitrogen source of tempeh flour produced the highest amylase, with mineral MgSO4.7H2O and 0,05% mineral concentration of 0,0084±0.0014 U/mL, and specific activity of 0,0164±0.00 U/ mg.Item OPTIMASI SUHU KARBONISASI DAN WAKTU KONTAK ADSORPSI ARANG TEMPURUNG KELAPA (Cocos nucifera L.) TERHADAP Escherichia coli(Elfitra, 2023-08) Fauziah, Miftahul; Itnawita, Itnawita; Devi, SilveraCoconut shell (Cocos nucifera L.) is one of the materials that can be used as a good raw material for making charcoal. Charcoal can be used as an adsorbent for metals, non-metals, and organic compounds. In this study, the potential of coconut shell charcoal was seen from its ability to adsorb E.coli (Escherichia coli). This study aims to determine the optimum carbonization temperature and contact time of coconut shell charcoal adsorption of E.coli. The coconut shell charcoal used is charcoal with a size that passes 50 mesh and is retained in 100 mesh. Surface area, pore volume, and pore radius were analyzed using a surface area analyzer with the BET-BJH method. Coconut shell charcoal morphology was analyzed using SEM. Based on the results of the BET obtained surface area of 12.8057 m2/g, a pore radius of 1.60979 nm, and a pore volume of 2.5 x 10-3 cc/g. The optimum adsorption conditions were obtained at the optimum carbonization temperature of 500⁰C and optimum contact time of 5 hours with an average amount of E.coli adsorbed of 5.68 x 108 cells/mL and adsorption percentage of E.coli of 71.2%.Item PENENTUAN KADAR TOTAL FLAVONOID DAN FENOLIK DARI TUMBUHAN PAKU SAYUR (Diplazium esculentum)(Elfitra, 2023-07) Rambah, Tiara Mulyani; Devi, Silvera; Hendra, RudiDiplazium esculentum is one of the species belonging to the Athyriaceae family which can be used as a medicinal plant. D. esculentum fern samples were extracted by maceration method using methanol solvent and followed by partitioned extract with two solvents namely n-hexane and ethyl acetate. Total flavonoid content was determined using the aluminium chloride colorimetric (AlCl3) method and total phenolics using the Folin-Ciocalteu method. The results of this study showed that the total flavonoids and phenolics content from ethyl acetate extract were higher than water extract with values of 149.90 ± 27.20 μg QE/g FW and 121.25 ± 8.29 μg GAE/g FW respectively.Item Produksi Kitinase Trichoclerma Asperellum Tnc52 Dan Tnj63 Galur Lokal Riau Menggunakan Limbah Kulit Udang(2015-07-08) Nurulita, Yuana; T. Nugroho, Titania; Devi, SilveraKitin merupakan polimer kedua terbanyak di alam setelah selulosa serta merupakan komponen penyusun tubuli serangga, udang, kepiting, ciimi-cumi, dan artropoda lainnya, serta bagian dari dinding sel kebanyakan fungi dan alga. Setiap tahun dari perairan (laut) dihasilkan sekitar lO" ton kitin, namun kurang dari 0,1% yang dimanfaatkan kembali. Proses degradasi kitin dapat menggunakan biokatalisator yaitu enzim yang tentunya lebih murah, aman, dan cepat jika dibandingkan dengan proses kimiawi tanpa enzim. Proses degradasi kitin menggunakan kitinase. Genus jamur Trichoderma sp. merupakan fungi yang mampu menghasilkan enzim kitinase (Loritto et al., 1993). Meneliti spesies galur ini adalah penting terutama galur lokal Riau T. asperelliim TNC52 dan TNJ63 dari segala aspek pemanfaatannya dalam menghasilkan kitinase. Penelitian ini bertujuan untuk menguji limbah cangkang udang laut dan sungai juga kitin IPB sebagai pembanding dalam memproduksi kitinase dari jamur T. asperellum lokal Riau dengan berbagai konsentrasi substrat yaitu 0,2; 0,5; 1; dan 2,5%. Kitinase yang dihasilkan diuji aktivitasnya dalam mendegradasi kitin koloidal SIGMA dan gula pereduksi yang dihasilkan dimonitor dengan nelson somogyi. Protein kitinase yang dihasilkan ditentukan kadar proteinnya untuk menentukan aktivitas spesifik kitinase.Item PRODUKSI SELULASE DARI BAKTERI ENDOFIT Pseudomonas stutzeri LBKURCC 54 DAN LBKURCC 59 MENGGUNAKAN SELULOSA POPOK BAYI SECARA FERMENTASI PADAT(2016-05-16) Amelia, Silfi; Devi, Silvera; Dahliaty, AndiDisposable diapers are made of cotton containing cellulose and can be used for the production of cellulase by solid state fermentation. Cellulase is an enzyme that can hydrolyze the β-1,4 glicosidic of cellulose and produce glucose. Cellulase can be produced by many microorganisms include endophytic bacteria Pseudomonas stutzeri LBKURCC 54 and LBKURCC 59 at fermentation time of 10, 20, 30, 40 and 50 days. The activity of cellulase was determined by the Nelson-Somogyi method. The results showed that highest cellulase activity of LBKURCC 54 was occurred at the 40th day of fermentation time (1.900 ± 0.346) x 10-3 U/mL, while the highest cellulose activity of LBKURCC 59 was occurred at the 30th day of fermentation time (3.530 ± 3.415) x 10-3U/mL.Item SELEKSI 16 ISOLAT BAKTERI ENDOFIT PENGHASIL SELULASE DARI AKAR MANGROVE Bruguiera sp(2021-01) Sari, Raja Putri Indah; Devi, Silvera; Itnawita, ItnawitaCellulase enzymes are enzymes that have many benefits both in vitro and in vivo. In vitro, this enzyme is used as a biocatalyst for certain reactions in industry, while in vivo it can be used to convert cellulose waste into products of economic value. In this study, 16 isolates were selected from 47 endophytic bacterial isolates from the mangrove roots of Bruguiera sp, which are a collection of the Biochemistry Laboratory of FMIPA UNRI. The selection was carried out in selective solid media containing 1% carboxyl methyl cellulose (CMC). The results of the selection of 16 endophytic bacteria from mangrove roots Bruguiera sp in 1% selective medium CMC obtained 15 live isolates because they were able to produce extracellular cellulase enzymes and 1 non-living isolate.Item SELEKSI 24 ISOLAT BAKTERI ENDOFIT SEBAGAI PENGHASIL SELULASE DARI AKAR MANGROVE Ceriops tagal (Perr) C. B. Rob(2020-01) Syari, Alfika; Devi, Silvera; Itnawita, ItnawitaEndophytic bacteria from mangroves have the potential to produce cellulase enzymes. The application of cellulase enzymes in vivo and in vitro is relatively large, so that research to obtain isolates capable of producing cellulase enzymes with high activity is still ongoing. Collection of 48 isolates from local strains of mangrove roots Ceriops tagal (Perr) C. B. Rob. (C. tagal) by Biochemistry Laboratory, Department of Chemistry Faculty of Mathematics and Natural Sciences, University of Riau (FMIPA UNRI) its potential to produce cellulase is unknown. In this study, only 24 isolates were selected from 48 isolates qualitatively using Carboxymethyl Cellulose (CMC) 1% selective solid media. The selection results showed that 22 other isolates were alive because they were able to produce extracellular cellulase enzymes and only 2 isolates could not live.Item SELEKSI DAN UJI AKTIVITAS SELULASE ISOLAT BAKTERI ENDOFIT LBKURCC 364 s/d 391 DARI AKAR MANGROVE KABUPATEN BENGKALIS(Elfitra, 2022-04) Haryani, Lia; Devi, Silvera; Itnawita, ItnawitaThe Laboratory of Enzyme, Fermentation and Biomolecular Research, Faculty of Mathematics and Natural Sciences, Riau University has a collection of endophytic bacterial isolates from Mangrove roots in Sei Pakning Jetty and Tenggayun Beach, Bengkalis Regency, Riau. This isolate is thought to have the potential to produce an area of enzyme, therefore this study aimed to select 28 isolates of cellulase-producing bacteria using 1% CMC selective solid media, while for the production of cellulase enzymes in liquid media. Testing of cellulase enzyme activity was carried out using the 3,5-Di Nitro Salissilic Acid (DNS) method. The enzyme activity data obtained were then tested statistically using the Duncan's Multiple Range Test (DMNRT) method at a significance level of 5% and Principal Component Analysis (PCA). The results of the selection of 28 isolates of endophytic bacteria from the roots of the Bengkalis Regency on 1% CMC selective solid media obtained 26 isolates that were alive because they produced cellulase enzymes and 2 were dead. The highest cellulase activity of the 13 crude extracts tested was LBKURCC388 isolate (487±105) x 10-4 U/mL mangrove species Bruguiera sp. which is in cluster 1, and the lowest activity is isolate LBKURCC365 mangrove plant species Ceriops tagal (Perr.) C.B. Rob. with an activity value of (72±39) x 10-4 U/mL in the 4th cluster.Item SELEKSI DAN UJI AKTIVITAS SELULASE ISOLAT BAKTERI ENDOFIT LBKURCC 392 s/d 418 DARI AKAR MANGROVE KABUPATEN BENGKALIS(Elfitra, 2022-04) Prananda, Shyntia Sukma; Devi, Silvera; Itnawita, ItnawitaEndophytic bacteria from mangrove roots have the potential to produce cellulase enzymes. In this study, 27 isolates of endophytic bacteria from mangrove roots were selected from the collection of the Enzyme, Fermentation and Biomolecular Research Laboratory, Department of Chemistry, FMIPA UNRI. The selection was carried out on selective solid media of carboxyl methyl cellulose (CMC) 1%, while the crude extract enzyme activity was determined by the 3,5-dinitrosalicylic acid (DNS) method. The results of the selection of 27 isolates of endophytic bacteria from the Magrove roots of Bengkalis Regency on 1% CMC selective solid media obtained 22 live isolates because they were able to produce the cellulase enzyme and 5 isolates not alive. Statistical analysis with DNMRT at a significance level of 5% and PCA with the highest activity in the plant species Ceriops tagal (Perr.) C.B. Rob (LBKURCC 409) is (489 ±137) x 10-4 U/mL in 1st cluster and the lowest activity was in plant species Bruguiera sp. (LBKURCC 393) with an activity value of (28 ±17) x 10-4 U/mL in the 4th cluster.Item Seleksi Kapang Pektinolitik Termotoleran Dari Kulit Jeruk Dan Tanah Di Kebun Jeruk(wahyu sari yeni, 2017-09-25) Martina, Atria; Devi, Silvera; Roza, Rodesia MustikaEnzim pektinase sangat penting bagi industri makanan dan kimia untuk pemrosesan material tumbuhan, industri jus, tekstil, kulit, anggur dan lain-lain. Penelitian ini bertujuan untuk menyeleksi kapang pektinolitik termotoleran dan kemampuannya menghasilkan pektinase. Teknik inokulasi langsung digunakan dengan menginokulasikan kulit jeruk yang telah membusuk dan tanah dari kebun jeruk ke medium yang mengandung pektin. Kultur diinkubasi pada suhu 50oC selama 5-7 hari. Isolat diseleksi terhadap kemampuan menghasilkan enzim pektinolitik. Zona bening yang yang terbentuk sekitar koloni berhubungan dengan aktivitas enzimatik. Aktivitas pektinolitik setiap isolat dihitung sebagai rasio diameter zona bening terhadap terhadap diameter koloni. Sebanyak 7 dari 8 isolat menghasilkan zona bening yaitu Aspergillus sp1, Aspergillus sp2, Aspergillus candidus, Aspergillus fumigatus TT, Aspergillus fumigatus KK, and Aspergillus fumigatus KP. Aktivitas pektinolitik yang paling tinggi diperoleh dari Aspergillus sp2 KP dengan rasio 2,2Item UJI ANTIDIABETES EKSTRAK ETIL ASETAT TUMBUHAN PAKU KELAKAI (Stenochlaena palustris)(Elfitra, 2023-06) Prananda, Adam; Hendra, Rudi; Devi, SilveraKelakai, also known as Stenochlaena palustris, is a fern that is commonly used as a medicinal plant for its antioxidant, antimicrobial, antianemia, and antidiabetic properties. The purpose of this study was to observe the activity of extracts and ethyl acetate fractions of S.palustris in its ability as inhibitor of the α-glucosidase enzyme. A plant is said to have good antidiabetic activity if it has an IC value50 below 100 μg/mL. According to the results of the in vitro antidiabetic test α-glucosidase inhibitory assay on the α-glucosidase enzyme performed, the ethyl acetate extract has an IC value50 of 37.57 μg/mL. Meanwhile, for the extraction results of ethyl acetate extract S.palustris using the vacuum liquid chromatography (VLC) method, 5 fractions were produced and only fractions 5 had good anti-diabetic activity, which had an IC value50 141,31 μg/mL.