Browsing by Author "Elfina, Dewi"

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    ISOLASI DAN KARAKTERISASI FUNGI ENDOFIT DARI KULIT BUAH MANGGIS (Garcinia mangostana L) SEBAGAI ANTIMIKROBA TERHADAP Candida albicans, Staphylococcus aureus DAN Escherichia coli
    (2014-03-28) Elfina, Dewi; Martina, Atria; Roza, Rodesia Mustika
    Garcinia mangostana Linn is one of the popular local plants that has been used as medicinal herb. Extract of Garcinia mangostana has been reported having anti-inflammatory, anti-tumour, anti-oxidant and antimicrobial activities. Bioactive compounds from extract of mangosteen fruit husk were thought to produce by endophytic fungi. The purpose of this research were to isolate and characterize endophytic fungi from mangosteen fruit husk that potential to produce antimicrobial compound. Antimicrobial activity was determined by measuring the inhibition zone with Agar Disc and Kirby-Bauer assay methods using pathogenic microbes i.e. Candida albicans, Staphylococcus aureus and Escherichia coli. Eleven of twenty isolates endophytic fungi which were obtained from mangosteen fruit husk were known to have antimicrobial activities. Endophytic fungi producing antimicrobial belongs to genus Aspergillus, Penicillium, Trichoderma, Alternaria and Fusarium. Endophytic fungi having the highest antimicrobial activities in agar disc assay were Penicillium sp.1KMA, Aspergillus sp.5KMR, Aspergillus sp.1KMA, Fusarium sp.KMR and Aspergillus sp.3KMB. Endophytic fungi having the highest antimicrobial activities in Kirby-Bauer assay was Penicillium sp.1KMA. Antimicrobial activities of Penicillium sp.1KMA was significantly different with the other four endophytic fungi