Browsing by Author "F, Bernadeta Leni"

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    Biomassa Nitrogen (N) Mikroba dan Aktivitas Urease Tanah di Cagar Biosfer Giam Siak Kecil-Bukit Batu: Sebagai Status Hara Nitrogen
    (2013-03-04) Komariah, Siti; F, Bernadeta Leni; Zul, Delita
    Giam Siak Kecil-Bukit Batu Biosphere Reserve (GSK-BB) is one of the peatland ecosystem that most of the regions have been converted into oil palm plantations, industrial forest plantation, and settlement. Environmental conditions disrupted by land conversion will affect the population, diversity and activity of soil microbes. This research aim was to analyze the impact of land use changes to nitrogen fixation bacterial cells number, microbial nitrogen biomass and urease activity in peatlands. Nitrogen fixation bacterial cells number was calculated by using a Total Plate Count (TPC) method, concentration of microbial N biomass was determined by using Chloroform Fumigation Extraction (CFE) and rate of urease activity was determined by quantifying ammonia released during an incubation by adding buffer solution. The result of physical and chemical characteristic of the soil ranged from 3.5 for pH, temperature 28.25-31.25oC, dry weight 21.20-42.86%, water content 63.2- 78.8% and bulk density 0.10-0.34 g/cm3. Cells number of nitrogen fixation symbiotic bacteria ranged from 3⋅2-4⋅9⋅103 CFU/g soil and cells number of nitrogen fixation non- symbiotic bacteria ranged from 3⋅2-9⋅3⋅102 CFU/g soil. Microbial N biomass varied ranging from 1.15-4.29 μg N/g soil and urease activity varied ranging from 3.66-39.28 μg NH4+/g dry weight soil/h. Based on the results obtained, it can be described that the soil quality in the GSK-BB Biosphere Reserve is still capable enough to support the sustainability of soil biological processes
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    ENUMERASI TOTAL POPULASI MIKROBA TANAH GAMBUT DI TELUK MERANTI KABUPATEN RIAU
    (2014-03-28) Fitria, Rahmi; Zul, Delita; F, Bernadeta Leni
    Teluk Meranti is one of the peatland area in Riau province. Most of these lands have been changed into palm oil plantation, timber plantation, agricultural area and settlement. The aim of this research was to analyze the impact of land use changes on soil physical-chemical characteristics and microbial cell number. Soil samples were taken from eight different locations, namely primary forest as control, secondary forest, rubber plantation (15 monthsyears old), rubber forest (40-60 years old), palm oil plantation (7-8 yearsold), acacia plantation (2-3 years old), corn field, and cassava field. Microbial cell number was determined by spread plate method, employing appropriate media for the growth of bacteria, fungi and actinomycetes. The results showed that the soil humidity, soil temperature, percentage of soil dry weight, water content, soil bulk density and pH ranged from 29,63-55,88%, 27-31,5o C, 14,9-35,5%, 64,9-85,1%, 0,16-0,39g/cm 3 and 3,63-4,00, respectively. The copiotrophic bacterial cell number ranged from 0,6x105-1,8x105 CFU/g soil where the highest population was at the palm oil plantation,whereas the oligotrophic bacterial cell number ranged from 0,5x105-1,4x105 CFU/g soil where the highest population was at the palm oil plantation. The population of fungi ranged from 0,4x105-1,0x105 CFU/g soil where the highest population was at the corn field. The population of actinomycetes ranged from 0,4x105-10,7x105 CFU/g soil where the highest population was at the palm oil plantation. Land use changes caused microbial cell number increased. The results indicated that land use changes influenced the microbial cell numbers
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    LAJU RESPIRASI TANAH DAN AKTIVITAS DEHIDROGENASE DI KAWASAN LAHAN GAMBUT CAGAR BIOSFER GIAM SIAK KECIL-BUKIT BATU
    (2013-04-25) A, Ria Nur; Zul, Delita; F, Bernadeta Leni
    Giam Siak Kecil-Bukit Batu Biosphere Reserve (GSK-BB) is one of the peatland areas in Riau province which consist of conservation and production areas. Most of these areas have been converted to oil palm plantations, timber plantations (HTI), agricultural and settlement areas. This research was aimed to analyze the impact of land use changes on soil physical-chemical characteristics, the number of bacterial cells, soil respiration rate, and soil dehydrogenase activity. Soil sample was taken from 6 different locations, primary forest as control, secondary forest, rubber plantation, rubber forest, andoil palm plantation (3 and 12 years old). The result indicated that the soil temperature, soil dry weight, water content, soil bulk density and pH, range from 28.25-31.250C, 15.1-36.1%, 63.9-84.9%, 0.10-0.34 g/cm3, and 3.5, respectively. The number of copiotrophic bacterial cells ranges from 3.0x105-5.4x105 CFU/g soil. The number of oligotrophic bacterial cells ranges from 3.3x105-4.6x105CFU/g soil. Furthermore, soil respiration rate ranges from 40.87-81.09 mg CO2/g soil/h for soil without glucose and 70.96-119.19 mg CO2/g soil/hfor glucose enriched soil. Soil dehydrogenase activity ranges from 16.25-158.20 μg formazan/g dry weight soil/h. The results also showed that land use changes influence soil respiration rate and soil dehydrogenase activity, therefore those parameters tested can serve as indicators for soil quality on peatland.
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    UJI POTENSI BAKTERI INDIGENUS TANAH GAMBUT DI CAGAR BIOSFER GIAM SIAK KECIL-BUKIT BATU SEBAGAI AGEN BIOFERTILIZER
    (2013-07-30) R, Nona Sri; Zul, Delita; F, Bernadeta Leni
    Giam Siak Kecil – Bukit Batu (GSK-BB) Biosphere Reserves is one of peat swamp forest located in Siak and Bengkalis, Riau Province which is habitat for many soil bacterias. Soil bacteria is known with its ability to release phosphorus (P) or provide nitrogen (N) for plant. The group functional of these bacteria can be used for biofertilizer agents. So far, the collection of peat soil bacteria isolated from GSK-BB Biosphere Reserves has not known their potential as biofertilizer agent. The bacteria potential assay was conducted by using a spot method using pikovskaya agar for phosphate solubilizing bacteria and yeast extract mannitol agar for nitrogen fixing bacteria. The number of isolates analyzed was 64 isolates. Based on the solubility index, isolate P410-51 showed the highest phosphatase activity with a value 8.6 and isolate HP4 10-2 showed the highest nitrogen fixing ability with the ratio clear zone diameter/colony diameter (Z/K) value was 4.11. Seventeen phosphate solubilizing bacteria and two nitrogen fixing bacteria were qualitatively potential in solubilizing organic phosphate and nitrogen fixation respectively. Those isolates can be employed as a component of biofertilizer.
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    UJI POTENSI BAKTERI SELULOLITIK ASAL TANAH GAMBUT DI CAGAR BIOSFER GIAM SIAK KECIL-BUKIT BATU DALAM MENDEGRADASI LIGNIN
    (2013-07-30) Hidayah; Zul, Delita; F, Bernadeta Leni
    Giam Siak Kecil-Bukit Batu (GSK-BB) Biosphere Reserve is one of peatland area in Riau Province. Peat soil contains high organic matter consisting of lignin, pectin, cellulose and hemicellulose. Lignin, a natural organic polymer, is abundant in nature, but it is difficult to degraded. The way to overcome this problem is by utilizing lignin degrading bacteria. So far, the ability of cellulolytic bacteria isolated from peat soil of GSK-BB Biosphere Reserve in lignin degradation is still unknown. Therefore, it is necessary to analyze the potential of cellulolytic bacteria collection in degrading lignin. Number of isolates analyzed is about 61 isolates. Semiquantitatively, ligninase enzyme activity by used of spot method employing guaiacol enrichment medium showed that 50 isolates are able to degrade lignin. As many as 12 isolates (24.0%) revealed high potential in lignin degradation based on median value test. Isolate PPA KBB 10-4B11 has the highest ligninase activity with a clear zone/colony diameter ratio value of 24.0.