Browsing by Author "Husnah, Ujang Al"

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    Isolasi Bakteri Selulolitik Asal Kulit Buah Durian di TPA Muara Fajar
    (2016-07-31) Hapsoh; Gusmawartati; Husnah, Ujang Al
    Selulosa merupakan salah satu bahan organik yang sulit sekali untuk dirombak,seperti misalnya pada kulit buah durian. Kulit buah durian merupakan limbah rumah tangga yang di buang sebagai sampah dan tidak memiliki nilai ekonomi. Kulit durian secara proporsional mengandung unsur selulosa yang tinggi (50-60%) dan kandungan lignin (5%) serta kandungan pati yang rendah. Karena memiliki kandungan selulosa yang tinggi, kulit durian memiliki kecenderungan adanya bakteri selulolitik yang berguna untuk merombak selulosa tersebut. Bakteri selulolitik memiliki kemampuan dalam menghidrolisis bahan-bahan dari alam yang mengandung selulosa menjadi produk yang lebih sederhana. Bakteri selulolitik memiliki peranan dalam proses dekomposisi selulosa secara enzimatik. Penelitian bertujuan untuk mengetahui bakteri yang dapat mendegradasi selulosa dan mengetahui potensi bakteri pendegradasi selulosa pada kulit buah durian. Sampel diperoleh dari TPA Muara Fajar. Isolasi dilakukan dengan metode pengenceran cawan tuang dan cawan gores menggunakan media yang mengandung Carboxymethyl cellulose (CMC). Pengamatan dilakukan secara morfologi dengan mengamati warna, bentuk, tepian dan elevasi bakteri. Hasil isolasi didapatkan 4 isolat (BKD MF1, BKD MF2, BKD MF3, BKD MF4) yang merupakan bakteri selulolitik asal kulit buah durian.
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    Microbial, Cellulolytic Isolation And Identification From Durian Leather Waste
    (2016-07-28) Hapsoh; Gusmawartati; Husnah, Ujang Al
    Isolation of microbial cellulolytic (cellulose decomposer) can grow naturally or intentionally given to accelerate the recast of organic material containing cellulose. This microbe has an important role in biogeochemical cycles and very responsive to the recycling of organic compounds. Durian’s peel is the household waste that being disposed as waste and has no economic value so that the recast of durian waste peel still occur naturally. Durian’s peel is one of the sources of cellulose which has not been utilized optimally. The research was aimed to obtain potentially cellulolytic microbial isolates derived from durian waste peel so that it can be utilized in recasting the cellulose either from the durian waste peel itselfs or other agriculture wastes. Cellulolytic microbes were isolated from durian shell waste that has naturally half decaying. The Microbes are grown in Carboxymethil microbial cellulose media (CMC) with some level of dilution. The Microbes’ isolation uses the scratch methods and pour-jelly methods. Cellulolytic microbial isolates was observed microscopically by gram staining. Microbes that can be isolated as much as seven isolates that four isolates of bacteria and three isolates of fungal. Based on results of the bacterial gram staining, two Gram negative bacteria and two Gram positive bacteria were obtained. The ability test of cellulolytic bacteria was tested qualitatively that seen from the resulting index cellulolytic bacterial isolates respectively of 3.9, 2.6, 2.8, 3.5 while the index cellulolytic generated by each of the fungi is 2.2, 1.8, 1.5. The larger the index cellulolytic generated, the greater the ability of microbes to degrade cellulose there