Browsing by Author "Jose, Christine"
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Item CHANGES IN PHENOLIC AND FLAVONOID CONTENT OF Coleus amboinicus TEA DURING STORAGE(2014-05-21) Jose, Christine; Nurhayati; ChainulfiffahColeus amboinicus plants were grown organically treated with three different foliar treatments (control, herb fermented plant extract and EM5). Green tea and black tea were produced from each treatment and stored for up to three months. All green teas have significant higher (P<0.05) phenolic and flavonoid contents compared to all black teas. Green tea produced from EM5 foliar treatment showed the highest phenolic and flavonoid content. Some changes of phenolic and flavonoid contents were observed in green teas and black teas during 3 months storage.Item Optimalisasi produksi enzim selulase isolat selulolitik yang diisolasi dari Sungai Siak(2013-04-16) Haryani, Yuli; Jose, Christine; dahliaty, andi; devi, silveraIsolates of S-16 and S-22 are some of the local strains producing cellulases that have been isolated from the Siak River water. Cellulase enzyme can be degrades of cellulosewaste containing that enable for the processing of waste to economically priced products such as glucose. In this research, optimization of the production of cellulase enzymes from isolates of S-16 and S-22 with a variety of media composition, temperature and concentration of carbon source production. The quantitative assay for enzyme activity was determined by Nelson Somogyi method with carboxymethyl of cellulose (CMC) as a substrate. The highest cellulase enzyme activity of S-16 obtained in a media production (KH2P04; MgS04.7H20; NaN03; CaC12; NaCl; CMC) of 88x10-3 U / mL, and the optimum concentration of source of carbon at 1% obtained for 50.36 xlO-3 U / mL, whereas of S-22 obtained in a media production (KH2P04; MgS04.7H20; FeS04.7H20; NaN03; KCI; CMC) of 14x10-3 U / mL, and the optimum concentration of sources of carbon at 1% obtained for 14 , 7x10-3 U / mL. Comparison of cellulase enzyme activity of isolate S-16 and S-22 by using Duncan test multiple range at level 5%.