Browsing by Author "Martina, Atria"

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    AKTIVITAS ANTIBAKTERI AKTINOMISETES LAHAN GAMBUT RIMBO PANJANG KAMPAR RIAU TERHADAP Staphylococcus aureus
    (2016-05-19) Rosandi, Gita Putri; Roza, Rodesia Mustika; Martina, Atria
    Potential actinomycetes in producing antibacterial compound has been widely studied. However, not so many information about antibacterial compound produced by local isolates actinomycetes from Riau despite its high diversity. The aim of this research is to determine potential of actinomycetes from Rimbo Panjang Peat, Kampar, Riau in producing antibacterial compounds against S. aureus. Agar disc method was used to test the antibacterial activity by measuring the inhibition zone. Furthermore, antibacterial compounds produced by isolated filtrate of actinomycetes, following five day fermentation, were tested using paper disc method. The result showed that 13 of 40 isolates which were tested using agar disc method had antibacterial activity against S. aureus with the highest inhibition zone was 18,45 mm (isolates KN 5.4). Meanwhile, only 6 of 13 isolates filtrate which were tested using paper disc method showed antibacterial activity with the highest inhibition zone of 16,16 mm (KN 5.4).
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    Aktivitas Ligninolitik Jamur Aphylloporales Strain Local Penghasil Lignin-Modifying Enzymes
    (wahyu sari yeni, 2017-09-07) Martina, Atria; Zul, Delita
    Jamur yang mempunyai aktivitas ligninolitik berpotensi diterapkan pada bidang bioteknologi seperti pada proses biopulping, biobleaching, dekolorisasi dan detoksifikasi limbah-limbah pabrik yang bersifat toksik dan sangat sulit terurai di lingkungan. Aktivitas ligninolitik dihasilkan oleh lignin-modifying enzymes yang terdiri dari lignin peroksidase, mangan peroksidase, dan lakase. Penelitian tentang aktivitas ligninolitik terhadap 14 jenis Aphyllophorales strain lokal yang berasal dari TNBT menggunakan prosedur lempeng agar pada medium agar “N-Limited-RBB” yang mengandung lignoselulosa dan lignin sintesis yaitu indulin AT. Seleksi isolat yang mempunyai aktivitas ligninolitik berdasarkan pembentuka zona perubahan warna pada medium selama inkubasi 6 hari pada suhu 37oC. Hasil penelitian menunjukan bahwa isolat yang mempunyai aktivitas ligninolitik pada medium mengandung indulin adalah Ganoderma sp, Stereum sp, dan AMS 27. Pada medium yang mengandung lignoselulosa, isolat yang mempunyai aktivitas ligninolitik adalah Ganoderma sp, Strereum sp, AMS 27, Polyporus sp2, Microporus sp1, dan Formes sp. Ganoderma sp merupakan isolat yang mempunyai aktivitas ligninolitik tertinggi pada kedua tipe lignin dengan diameter zona perubahan warna pada indulin AT adalah 8,71 cm dan pada lignoselulosa adala 9,71 cm.
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    Antifungal Spectra of Activity of Actinomycetes Strains Against Rhizoctonia solani
    (2017-08-07) Linda, Tetty Marta; Martina, Atria; Sinaga, Juni
    Biological control offers an enviromentally friendly alternative to the use of antifungal for controlling damping off diseases. A collection of about 24 actinomycetes strains from peat soil Siak-Riau was screened for the ability to produce metabolites that inhibit R. solani growth in vitro. Seven isolate showed strong in invitro antagonistic against R. solani in agar disc and well-difussion methods by producing extra cellular antifungal metabolites.
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    Biodegradasi Herbisida Atrazin Oleh Rhizobium Spp Indigenus Diisolasi Dari Nodul Akar
    (wahyu sari yeni, 2017-09-13) Martina, Atria; Zul, Delita
    Atrazin merupakan herbisida yang sering digunakan pada pertanian dan sering mengkontaminasi tanah dan air tanah akibat intensifikasi pertanian yang tidak terkontrol. Atrazin sangat toksik dan sukar terdegradasi dalam tanah. Beberapa jenis mikroba tanah mampu mendegradasi herbisida ini. Bakteri rhizobium yang hidup bebas di tanah dan daerah perakaran tanaman legum dapat mendegradasi atrazin. Penelitian ini bertujuan untuk memperoleh isolat Rhizobium spp serta menguji kemampuannya dalam mendegradasi herbisida atrazin. Rhizobium spp diisolasi dari nodul akar tanaman legum. Sampel diambil secara acak di tiga desa di Kec. Kampar. Masing-masing daerah dilakukan 5 pencuplikan dengan 3 kali pengulangan. Seleksi dilakukan 2 tahap. Tahap pertama menggunakan garam mineral yang mengandung atrazin 5 mg/l dengan waktu inkubasi satu minggu sedangkan tahap kedua dengan konsentrasi atrazin 10 mg/l diinkubasi 8 hari. Hasil penelitian menunjukkan pada konesentrasi atrazin 5 mg/l terdapat 22 isolat Rhizobium yang mampu tumbuh. Pada konsentrasi atrazin 10 mg/l terdapat 9 isolat yang membentuk zona perubahan warna dan berarti mampu mendegradasi atrazin. Rhizobium sp isolat RA 4.2 merupakan isolat yang mempunyai kemampuan paling cepat dan tertinggi dengan diameter zona perubahan warna 9,98 cm. Lima isolat mempunyai kemampuan degradasi terendah dan pembentukan zona perubahan warna yang lambat dengan diameter zona 0,6 cm
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    Degradasi Pewarna Azo Mordant Black 17 oleh Ganoderma sp. BTA1 Strain Lokal
    (wahyu sari yeni, 2017-09-07) Martina, Atria; Rahayu, Lila Kusuma; Fitriyanti
    Ganoderma sp. BTA1 local strain is a white rot basidiomycetes that have ligninolytic activities. This fungi was used for azo Mordant Black 17 (MB17) degradation. Their performance were evaluated at different Mordant Black 17 concentration and incubation time. To approve the ability of those isolates using N-limited medium and monitoring with specrofotometer. Idiophase of this isolate was 11th day. The experiment on MB 17, indicated that the Ganoderma sp BTA1 were resistent at 200 mg/l concentration of MB 17 with optimal result 150 ppm. The optimal concentration was used for further experiment. The result of this study indicated that the capacity of MB17 degradation was affected by incubation times. The degradation rate was greater at six days after idiophase with 95%.
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    Effectivenes of Vesicular-arbuscular Mycorrhiza and Bokashi on Mycorrhizal Infection and Seedling Growth of Mangosteen (Garcinia Mangostana L.)
    (wahyu sari yeni, 2017-09-25) Martina, Atria; Isda, Mayta Novaliza; Sartika, Endang; Hidayat, Asep
    Vesicular-Arbuscular mycorrhizal (VAM) fungi were co-inoculated with Bokashi to Garcinia mangostana to study the effectiveness of microsymbiont to the mycorrhizal infection and seddling of the plants. A factorial in Completely Randomized Design was employed in this study, with three VAM dosage (0,10,20 g/ seedling) and three bokashi (0,65 and 85 g/seedling). The result showed that VAM dosage 20 g/seedling without bokashi provided the best result on the growth of plants. The teratment had VAM infection could reach 100% bright root and less root hair. The VAM dosage 20 g/seedling without bokashi was significant to plant height and dry weight.
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    Eksplorasi Dan Uji Daya Hambat Aktinomisetes Asal Tanah Gambut Cagar Alam Giam Siak Kecil-Bukit Batu Riau Terhadap Bakteri
    (wahyu sari yeni, 2017-09-13) Roza, Rodesia Mustika; Linda, Tetty Marta; Martina, Atria
    Penelitian ini bertujuan untuk mendapatkan isolat aktinomisetes asal tanah gambut Cagar Alam Giam-Siak Kecil Bukit Batu Riau yang memiliki kemampuan daya hambat terhadap bakteri. Penelitian ini dilaksanakan di lapangan dan di laboratorium Mikrobiologi Jurusan FMIPA Universitas Riau mulai bulan Juli-Desember 2009. Lokasi pengambilan sampel terdiri atas empat lokasi yaitu : Lokasi 1 (Perkebunan Sawit dan Karet), Lokasi 2 (Hutan Tanaman Industri), Lokasi 3 (Hutan Paska Kebakaran) dan Lokasi 4 (Zona Inti). Penghitungan total mikroba dilakukan pada medium PCA (Plate Count Agar). Hasil penghitungan total mikroba diperoleh jumlah tertinggi pada lokasi 4 (zona inti) sebanyak 143 x 103 CFU /gram sampel tanah gambut dan terendah pada Lokasi 3 (Hutan Paska Kebakaran) sebanyak 39 x 103 CFU /gram sampel tanah gambut. Hasil isolasi aktinomisetes diperoleh total 22 isolat aktinomisetes, 2 isolat dari Lokasi 1 (Perkebunan Sawit dan Karet), 2 isolat dari Lokasi 2 (Hutan Tanaman Industri), 13 isolat dari Lokasi 3 (Hutan Paska Kebakaran) dan 5 isolat dari Lokasi 4 (Zona Inti). Warna koloni yang berhasil diisolasi dikelompokkan putih, krem, coklat, putih kecoklatan coklat kekuningan, dan kehijau-hijauan, permukaan bertepung, hampir keseluruhan berbentuk bulat, semua berkonsistensi lengket dan mengeluarkan bau serasah/ bau tanah. Isolasi dilakukan dengan menggunakan medium SCA (Starch Casein Agar). Pengujian kemampuan daya hambat terhadap bakteri uji (E.coli) diperoleh satu isolat (L3.9) memperlihatkan zona bening. Zona hambat yang terbentuk berdiameter 13,7 mm. Pengujian daya hambat terhadap bakteri dilakukan pada medium NA (Natrium Agar)
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    Eksplorasi Dan Uji Daya Hambat Aktinomisetes Asal Tanah Gambut Cagar Biosfir Giam Siak Kecil-Bukit Batu Riau Terhadap Bakteri Dan Jamur
    (wahyu sari yeni, 2018-08-23) Roza, Rodesia Mustika; Linda, Tetty Marta; Martina, Atria; Fahrizawati, Fahrizawati
    Cagar Biosfir Giam Siak Kecil-Bukit (Cagar Biosfir GSK-BB) merupakan hutan gambut dataran rendah. Tanah gambut ini merupakan habitat utama mikroba tersterial salah satunya aktinomisetes. Aktinomisetes memiliki kemampuan menghasilkan berbagai metabolit sekunder. Oleh karena itu, dilakukan penelitian yang bertujuan untuk mengeksplorasi aktinomisetes asal tanah gambut Cagar Biosfir GSK-BB dan melakukan uji daya hambat aktinomisetes terhadap bakteri dan jamur. Sebanyak 33 isolat aktinomisetes yang diisolasi dari sampel tanah gambut menggunakan metode pour plate dalam medium Strach Casein Agar, 2 isolat (GSK.5.7 dan GSK.5.10) mampu menghambat S. pyogenes dan 1 isolat (GSK.4.9) mampu menghambat E. Coli, 2 Isolat (GSK.4.3 dan GSK.4.5) mampu menghambat pertumbuhan F. oxysporum, dan 11 isolat (GSK.1.1, GSK.4.1, GSK.4.4, GSK.4.6, GSK.4.7, GSK.4.8, GSK.4.10, GSK.4.12, GSK.5.5, GSK.5.6, dan GSK.5.9) mampu menghambat pertumbuhan Ganoderma sp. BTA1.
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    Eksplorasi Dan Uji Daya Hambat Aktinomisetes Asal Tanah Gambut Cagar Biosfir Giam Siak Kecil-Bukit Batu Riau Terhadap Bakteri Dan Jamur
    (2017-09-11) Roza, Rodesia Mustika; Linda, Tetty Marta; Martina, Atria; Fahrizawati
    Cagar Biosfir Giam Siak Kecil-Bukit (Cagar Biosfir GSK-BB) merupakan hutan gambut dataran rendah. Tanah gambut ini merupakan habitat utama mikroba tersterial salah satunya aktinomisetes. Aktinomisetes memiliki kemampuan menghasilkan berbagai metabolit sekunder. Oleh karena itu, dilakukan penelitian yang bertujuan untuk mengeksplorasi aktinomisetes asal tanah gambut Cagar Biosfir GSK-BB dan melakukan uji daya hambat aktinomisetes terhadap bakteri dan jamur. Sebanyak 33 isolat aktinomisetes yang diisolasi dari sampel tanah gambut menggunakan metode pour plate dalam medium Strach Casein Agar, 2 isolat (GSK.5.7 dan GSK.5.10) mampu menghambat S. pyogenes dan 1 isolat (GSK.4.9) mampu menghambat E. Coli, 2 Isolat (GSK.4.3 dan GSK.4.5) mampu menghambat pertumbuhan F. oxysporum, dan 11 isolat (GSK.1.1, GSK.4.1, GSK.4.4, GSK.4.6, GSK.4.7, GSK.4.8, GSK.4.10, GSK.4.12, GSK.5.5, GSK.5.6, dan GSK.5.9) mampu menghambat pertumbuhan Ganoderma sp. BTA1.
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    Eksplorasi Dan Uji Daya Hambat Aktinomisetes Asal Tanah Gambut Cagar Biosfir Giam Siak Kecil-Bukit Batu,Riau Terhadap Jamur
    (2018-08-23) Roza, Rodesia Mustika; Linda, Tetty Marta; Martina, Atria; Fahrizawati, Fahrizawati
    Tanah merupakan habitat utama mikroba teresterial sekaligus sumber utama mikroba yang banyak digunakan dalam industri. Tiap-tiap tanah karena perbedaan sifat fisikokimianya menyebabkan pada masing-masing tempat memiliki kekhasan mikroba yang berbeda-beda. Crueger dan Crueger (1984), menjelaskan bahwa kelimpahan mikroba di dalam tanah bervariasi diantaranya genus Arthrobacter jumlah kelimpahan 5-60%, Bacillus 7-67%, Pseudomonas 3-15%, Agrobacterium 1-20%, Alcaligenes 2-12%, Flavobacterium 2-10% dan Actinomycetes 10-33%. Menurut Madigan, Martinko dan Parker (1994), aktinomisetes merupakan kelompok bakteri yang berbentuk filamen, Gram Positif dan membentuk cabang-cabang filamen (miselium) serta dapat hidup pada lingkungan yang beragam dan ekstrim. Aktinomisetes memiliki warna yang buram atau opak, tidak mengkilap, permukaan bertepung serta melekat kuat pada agar. Permukaan yang bertepung merupakan kumpulan hifa yang terdiri dari spora. Morfologi ini hanya terlihat pada aktinomisetes dewasa sedangkan pada koloni yang masih muda hanya terdiri dari hifa. Aktinomisetes dapat merubah warna medium karena memiliki warna yang mudah larut dalam air dan akan berdifusi ke dalam medium.Oskay, Tamer dan Azeri (2004) menjelaskan aktinomisetes mampu menghasilkan berbagai metabolit sekunder yang berbeda-beda seperti antibiotik, herbisida, pestisida, anti parasit dan enzim-enzim seperrti sellulosa dan xilanase. Dalam studi ini, sampel tanah akan dikoleksi dari berbagai habitat berbeda pada kawasan Cagar Biosfir Giam Siak Kecil-Bukit Batu Riau untuk dikaji keragaman aktinomisetesnya. Aktinomisetes yang diisolasi akan diuji daya hambatnya terhadap jamur.
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    EKSPLORATION OF GIAM SIAK KECIL-BUKIT BATU BIOSFEER RESERVE FOR CARBOLYTIC FUNGI
    (2014-05-21) Nugroho, TitaniaTjandrawati; Zul, Delita; Martina, Atria; Puspita, Fifi; Sepryani, Harni; Ismet, Rhahma Sari; Suhyana, Jajang; Utamy, WidyaRahmy
    GiamSiak Kecil-Bukit Batu (GSKBB) Biosphere Reserve in Riau Province, Indonesia, has a unique tropical peat swamp forest with peat depth reaching up to 20 meters deep. As a tropical peat swamp forest it is a potential source for carbolytic enzyme producing microbes. In this paper we present our exploration at two different sites of the GSKBB Biosphere Reserve forest, representing primary and secondary forest, for carbolytic fungi. Although the isolation media was design for the specific isolation of Trichoderma, we isolated carbolytic enzyme producers from the genus Trichoderma, PenicilliumandAspergillus. From the primary forest, a total of four cellulase and two chitinase producing fungi were isolated to purity. From the secondary forest, a total of two cellulase and one chitinase producing fungi were isolated to purity. Highest specific activity of cellulase was produced by a Penicillium secondary forest isolate. Highest specific activity of chitinase was produced by an Aspergillus primary forest isolate, with a total chitinase crude extract specific activity of 0.04 U/mg at 40oC, pH 5.5.
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    INOKULASI JAMUR ISOLAT LOKAL RIAU PADA SUBSTRAT SERBUK GERGAJI DAN AMPAS TEBU TERHADAP PERTUMBUHAN MISELIUM JAMUR TIRAM
    (wahyu sari yeni, 2019-07-15) Sakinah, Sakinah; Martina, Atria; Linda, Tetty Marta
    The growth of oyster mushrooms (Pleurotus ostreatus) requires a lignocellulose-containing substrate such as sawdust and baggase. The purpose of this study was to analyze the optimal formulation of a combination of sawdust and bagasse with which inoculated on the growth of oyster mushrooms mycellium the addition of local isolates Aspergillus fumigatus TT and Aspergillus sp II. The experiments were arranged in factorial complete randomized design with factor of substrate and isolate. The results showed mycelium growth was effected by the substrate. The highest growth rate of P. ostreatus mycelium was found in 30% sawdust + 70% bagasse with Aspergillus fumigatus TT (1.24 cm/day), 30% sawdust + 70% bagasse with A. fumigatus TT + Aspergillus sp II (1.38 cm/day) and 100% bagasse with A. fumigatus TT (1.39 cm/day). Combination of bagasse without of isolate in substrat result in a longer of primordium initition
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    INVENTARISASI AGARICALES DI KAWASAN ARBORETUM UNIVERSITAS RIAU PEKANBARU
    (2020-10) Zulham, Zulham; Martina, Atria
    Indonesia is a tropical country with a high diversity of macrofungi that plays an important role in environmental ecosystems, as food and medicine materials. This research aimed to explore the diversity of macroscopic fungi in the Arboretum area of Universitas Riau. This research used exploration methods; macrofungi were collected, characterized, and preserved. The results of the study obtained as many as 23 types of mushrooms of the Agaricales Order, consisting of 7 families. Marasmiaceae was the dominant family (8 species), most of the Marasmius species. This family was followed by Tricholomataceae 7 species, Lycoperdaceae 4 species, while Mycenaceae, Cortinariaceae, Pleurotaceae, Schizophyllaceae were found 1 species of fungus each.
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    INVENTARISASI JAMUR MAKRO PADA PERKEBUNAN KARET DESA TITIAN MODANG KOPAH, KABUPATEN KUANTAN SINGINGI
    (perpustakaan UR, 2021-10) Roza, Wilia Mai; Martina, Atria
    Indonesia has high diverse macrofungi species, which have important benefits in food with potential as medicine for humans. This study aimed to explore the biodiversity of macrofungi and its potential use in the Rubber Plantation of Titian Modang Kopah village, Kuantan Singingi. This study used an exploration method, and descriptive analysis. Twenty macrofungi were obtained included 20 general, 11 families, and 2 phyla (Ascomycota and Basidiomycota). The Order found is Polyporales, Agaricales, Poriales, Auriculariales, and Xylariales. Polyporales were dominated (8 species), namely Heterobasidion annosum, Lentinus sp. 1., Trametes suaveolens, T. gibbosa, T. versicolor, Trametes sp. 1., Polyporus sp. 1., Pycnoporus sanguineus, dan Spesies 1.
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    Isolasi Dan Aktivitas Antimikroba Aktinomisetes Asal Tanah Rizosfer Cagar Biosfer Giam Siak Kecil-Bukit Batu Riau
    (wahyu sari yeni, 2018-08-23) Roza, Rodesia Mustika; Linda, Tetty Marta; Martina, Atria; Haloho, Lindasari
    The aims of study are to isolate actinomycetes from rhizosphere soil, to determine the ability of actinomycetes to inhibit the growth of bacteria (Streptococcus pyogenes and Escherichia coli) and fungi (Fusarium oxysporum and Ganoderma BTA1). A total of 15 actinomycetes strains were recovered from rhizosphere soil samples using pour plate method with Starch Casein Agar (SCA). The results showed that 7 isolates (TBR1, TBR2, TBR3, MHGI1, MHG3, MHG4, FPR4) were active againts all microbial targets, 1 isolate (MHG2) was active againts S. pyogenes, F. oxysporum, and Ganoderma sp BTA1, 2 isolates (MHG5, FPR3) were active againts S. pyogenes, E. coli and F. oxysporum, and Ganoderma sp BTA1, 1 isolates (FPR1) was active againts S. pyogenes, E. coli and F. oxysporum, 1 isolate (FPR5) was only active againts Ganoderma sp BTA1, 2 isolates (TBR4, FPR2) were only active againts E. coli, and isolates (AKA1) was not active againts all microbial targets.
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    Isolasi Dan Aktivitas Antimikroba Aktinomisetes Asal Tanah Rizosfer Cagar Biosfer Giam Siak Kecil-Bukit Batu Riau
    (2017-10-11) Roza, Rodesia Mustika; Linda, Tetty Marta; Martina, Atria
    The aims of study are to isolate actinomycetes from rhizosphere soil, to determine the ability of actinomycetes to inhibit the growth of bacteria (Streptococcus pyogenes and Escherichia coli) and fungi (Fusarium oxysporum and Ganoderma BTA1). A total of 15 actinomycetes strains were recovered from rhizosphere soil samples using pour plate method with Starch Casein Agar (SCA). The results showed that 7 isolates (TBR1, TBR2, TBR3, MHGI1, MHG3, MHG4, FPR4) were active againts all microbial targets, 1 isolate (MHG2) was active againts S. pyogenes, F. oxysporum, and Ganoderma sp BTA1, 2 isolates (MHG5, FPR3) were active againts S. pyogenes, E. coli and F. oxysporum, and Ganoderma sp BTA1, 1 isolates (FPR1) was active againts S. pyogenes, E. coli and F. oxysporum, 1 isolate (FPR5) was only active againts Ganoderma sp BTA1, 2 isolates (TBR4, FPR2) were only active againts E. coli, and isolates (AKA1) was not active againts all microbial targets.
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    ISOLASI DAN KARAKTERISASI FUNGI ENDOFIT DARI KULIT BUAH MANGGIS (Garcinia mangostana L) SEBAGAI ANTIMIKROBA TERHADAP Candida albicans, Staphylococcus aureus DAN Escherichia coli
    (2014-03-28) Elfina, Dewi; Martina, Atria; Roza, Rodesia Mustika
    Garcinia mangostana Linn is one of the popular local plants that has been used as medicinal herb. Extract of Garcinia mangostana has been reported having anti-inflammatory, anti-tumour, anti-oxidant and antimicrobial activities. Bioactive compounds from extract of mangosteen fruit husk were thought to produce by endophytic fungi. The purpose of this research were to isolate and characterize endophytic fungi from mangosteen fruit husk that potential to produce antimicrobial compound. Antimicrobial activity was determined by measuring the inhibition zone with Agar Disc and Kirby-Bauer assay methods using pathogenic microbes i.e. Candida albicans, Staphylococcus aureus and Escherichia coli. Eleven of twenty isolates endophytic fungi which were obtained from mangosteen fruit husk were known to have antimicrobial activities. Endophytic fungi producing antimicrobial belongs to genus Aspergillus, Penicillium, Trichoderma, Alternaria and Fusarium. Endophytic fungi having the highest antimicrobial activities in agar disc assay were Penicillium sp.1KMA, Aspergillus sp.5KMR, Aspergillus sp.1KMA, Fusarium sp.KMR and Aspergillus sp.3KMB. Endophytic fungi having the highest antimicrobial activities in Kirby-Bauer assay was Penicillium sp.1KMA. Antimicrobial activities of Penicillium sp.1KMA was significantly different with the other four endophytic fungi
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    ISOLASI DAN KARAKTERISASI JAMUR INFEKSI KUKU
    (Elfitra, 2023-02) Ulfia, Mirza; Martina, Atria
    Onychomycosis is a fungal infection of the nail plate caused by dermatophytes fungi, non-dermatophytes fungi and yeast. Onychomycosis causes infection of nails, they become thickening and separation from the nail bed easily. The factor that causes nails to become infected by the fungus is frequently direct contact with watery and dirty environment. The purpose of this study was to determine the presence of fungal infection Aspergillus sp. on the nails of patients infected with the fungus. This study is descriptive research. Samples were taken from patient's nails and the observation was carried out at the Biology Laboratory, Faculty of Mathematics and Natural Sciences, University of Riau. Analysis was carried out through the direct observation and fungal culture methods. Results of the identification showed that the nail samples were positively infected by Tinea unguium (nail fungus). In addition, fungal cultures were positively infected by the fungus Aspergillus sp.
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    ISOLASI DAN SELEKSI BAKTERI ASAM LAKTAT DARI YOGHURT KEMASAN YANG BERSIFAT ANTIBAKTERI TERHADAP Escherichia coli DAN Salmonella typhi
    (2014-03-28) Yuliana, Ike; Roza, Rodesia M.; Martina, Atria
    Yoghurt is a fermented product of a group of lactic acid bacteria (LAB) on milk that has been pasteurized. LAB is a group of bacteria that are able to convert carbohydrates (glucose) into lactic acid. LAB produces metabolites that inhibit the growth of pathogenic bacteria. This study aimed to isolate LAB from yoghurt packaging with its inhibitory against bacterial pathogens Escherichia coli and Salmonella typhi. LAB was isolated from 9 samples with 3 brand products and 3 different flavors. Calculation of the total population to the overall LAB samples was obtained between 6.4 × 106 cfu/ml to 2.1 × 107 cfu/ml. In this study, inhibition test of pathogenic bacteria E. coli and S. typhi used samples yoghurt. The method used is the well-agar diffusion. The most effective yoghurt in inhibiting E. coli was yogurt Ab with diameter of inhibiting zone 23.55 mm, while the yogurt sample that had the highest inhibitory zone which is 19.93 mm for S.typhi was yoghurt Ap
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    ISOLASI DAN SELEKSI BAKTERI ASAM LAKTAT DARI YOGHURT PRODUKSI INDUSTRI RUMAH TANGGA DI PEKANBARU YANG BERSIFAT ANTIBAKTERI TERHADAP Escherichia coli DAN Salmonella typhi
    (2014-03-28) Yani, Lili; Roza, Rodesia Mustika; Martina, Atria
    The occurance of Lactic Acid Bacteria (LAB) in yoghurt produced by home industry in Pekanbaru as probiotic potentially improve intestinal physiological function, useful for health as well as prevention of the growth of pathogenic bacteria. The purposes of this study were to calculate the total LAB population of yoghurt with the effect of mango and strawberry juice during different storage process, and also to determine the ability of antibacterial activity against Escherichia coli and Salmonella typhi. The population of LAB in yoghurt was 4,0 × 107 – 2,9 × 108 CFU/ml. Antimicrobial activity assay used the well diffusion method. The mango juice yoghurt incubated in 7 day was the most effective for Escherichia coli with the inhibition zone 27,1 mm. While the mango juice yoghurt incubated in 3 day was the most effective for Salmonella typhi with inhibition zone 29,9 mm