Browsing by Author "Nurulita, Yuana"

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    AKTIVITAS ANTIBAKTERI DARI FRAKSI EKSTRAK ETIL ASETAT KO-KULTUR Penicillium sp. LBKURCC34 DAN Staphylococcus aureus
    (Elfitra, 2022-01) Nurhidayati, Rahmadanis; Nurulita, Yuana; Yuharmen, Yuharmen
    Penicillium sp. LBKURCC34 has the ability to produce antibacterial activity by single and co-culture fermentation. To increase diversity of chemical compounds, in this study, Penicillium sp. LBKURCC34 was fermented by co-culture with the addition of Staphylococcus aureus on day 3rd for 14 days incubation. To obtain active fractions as antibacterial activity from the ethyl acetate extract against Escherichia coli and Staphylococcus aureus, fractionation using Vacuum Liquid Chromatography (VLC) was applied. It resulted in 15 fractions, the highest antibacterial activity against bacteria E. coli in F3A, F6A, and F6B fractions and against bacteria S. aureus in F3A, F4, and F5 fractions with the best activity against S. aureus were F3A that contained nonpolar compounds.
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    AKTIVITAS ANTIMIKROBA EKSTRAK KO-KULTUR ISOLAT JAMUR LOKAL RIAU Penicillium sp. LBKURCC34 DAN Penicillium sp. LBKURCC30
    (Elfitra, 2023-09) Rahmat, Riska Alifia; Nurulita, Yuana
    Penicillium sp. LBKURCC34 and LBKURCC30 are fungi that have the potential to produce secondary metabolites that have many activities. This study was conducted to produce secondary metabolites using co-culture fermentation. Extraction of secondary metabolites was carried out using ethyl acetate (EtOAc), butanol (BuOH) and acetone (Ace). Antimicrobial activity test of these three extracts was conducted using disc diffusion method. Based on the clear zone area at concentrations of 2000 and 200 μg/disk, the three extracts did not have antibacterial activity against the four pathogenic microbes. Antifungal test results at a concentration of 2000 μg/disk these three extracts have antifungal activity against C. albicans fungi, while at a concentration of 200 μg/disk only EtOAc and BuOH extracts have antifungal activity.
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    AKTIVITAS ANTIOKSIDAN FERMENTASI KO-KULTUR ISOLAT JAMUR LOKAL RIAU Penicillium sp. DAN Trichoderma sp.
    (Elfitra, 2022-07) Ferani, Aisya; Nurulita, Yuana; Rafi, Mohamad
    Local isolates of the Riau fungus Penicillium sp. LBKURCC34, Trichoderma asperellum LBKURCC1 and Trichoderma asperelloides LBKURCC2 are well-known potential producers of secondary metabolites with several bioactivities, such as antioxidants. This study aims to determine the antioxidant activity of the co-culture extracts of these local isolates of Riau. Production of the compounds by co–culture fermentation and extraction of their fermentation media, Penicillium sp. LBKURCC34 and Trichoderma asperellum LBKURCC1 (Co-Culture A), Penicillium sp. LBKURCC34 and Trichoderma asperelloides LBKURCC2 (Co-Culture B) and the three fungi (Co-Culture C) were performed using organic solvents butanol (BuOH), ethyl acetate (EtOAc) and acetone (Ace). The antioxidant activity was analyzed by DPPH. Based on the research, co-cultured extracts of Butanol C and Acetone C were the most active antioxidant activity with IC50 values of 48.428 ± 3.956 μg/mL and 43.331 ± 3.994 μg/mL respectively.
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    AMPLIFIKASI GEN CaM Penicillium sp. LBKURCC34 SECARA POLYMERASE CHAIN REACTION
    (Elfitra, 2022-06) Risdarenuadie, Septiani; Nugroho, Titania Tj.; Nurulita, Yuana
    The fungal isolate Penicillium sp. LBKURCC34 is one of the isolates that has been successfully isolated from peat soil in the core zone of the Giam Siak Kecil-Bukit Batu Biosphere Reserve (GSKBB), Riau Province. Fungal isolates have the potential to produce secondary metabolites with antimicrobial activity, and it is important to correctly know the species of the isolate for inventory reasons, ease of secondary metabolite isolation, purification and utilization. In this study, as a first step toward the molecular identification of Penicillium sp. LBKURCC34, the fungal chromosomal DNA was amplified in the CaM region using the Polymerase Chain Reaction (PCR) method. A horizontal electrophoresis process was carried out to detect the success of the amplification process and calculate the molecular weight or length of the DNA products. A PCR product corresponding to CaM amplified region was successfully obtained after 35 cycles with the following conditions: 45 seconds of template denaturation at 94oC, 45 seconds of primer annealing at 55oC, and 1 minute of primer elongation at 72oC. The final products were subjected to a final elongation for 10 minutes at 72o C. The PCR product of the CaM amplified region of Penicillium sp. LBKURCC34 had a DNA length of 785 bps.
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    EKSTRAKSI DNA BAKTERI ENDOFIT Enterobacter cloacae LBKURCC146 DAN LBKURCC147
    (wahyu sari yeni, 2019-07-22) Alamanda, Gea Vini; Roslim, Dewi Indriyani; Nurulita, Yuana
    LBKURCC146 and LBKURCC147 are endophytic bacteria that isolated from Harupat ferns stem in Pekanbaru, Riau that resistant to acidic pH. The purpose of this study was to extract the DNA of endophytic bacteria Enterobacter cloacae LBKURCC146 and LBKURCC147. DNA of LBKURCC146 and LBKURCC147 bacteria were isolated after culturing for 24 hours inccubation using Geneaid Kit. The size of bacterial DNA obtained from the isolation results is above 10037 pb. DNA can be used for further step such as amplification (PCR).
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    FRAKSINASI EKSTRAK ETIL ASETAT DAN UJI KROMATOGRAFI LAPIS TIPIS DARI FERMENTASI KO-KULTUR Penicillium sp. LBKURCC34 DAN Staphylococcus aureus
    (Elfitra, 2022-03) Krisma, Debby Dwi; Yuharmen, Yuharmen; Nurulita, Yuana
    Penicillium sp. LBKURCC34 is a fungus that can produce bioactive secondary metabolites so that it can be used as a candidate for antimicrobial compounds producer. This study aimed to initiate separation of antimicrobial compounds from secondary metabolites of co-culture fermentation Penicillium sp. LBKURCC34 and Staphylococcus aureus. The ethyl acetate extract of the co-culture fermentation was obtained from liquid-liquid extraction of fermentation media and ethyl acetate solvent. That crude extract was then fractionated using vacuum liquid chromatography (VLC). Thin layer chromatography (TLC) test was performed on crude extract and all fractions with ethyl acetate – n-hexane (7:3) and ethyl acetate – methanol (3:1) as eluents. Fractionation of crude extract from vacuum liquid chromatography produced 15 fractions with different spot patterns seen under 254 nm UV lamp.
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    FRAKSINASI EKSTRAK ETIL ASETAT DAN UJI KROMATOGRAFI LAPIS TIPIS DARI FERMENTASI KO-KULTUR Penicillium sp. LBKURCC34 DAN Staphylococcus aureus
    (Elfitra, 2022-02) Krisma, Debby Dwi; Yuharmen, Yuharmen; Nurulita, Yuana
    Penicillium sp. LBKURCC34 is a fungus that can produce bioactive secondary metabolites so that it can be used as a candidate for antimicrobial compounds producer. This study aimed to initiate separation of antimicrobial compounds from secondary metabolites of co-culture fermentation Penicillium sp. LBKURCC34 and Staphylococcus aureus. The ethyl acetate extract of the co-culture fermentation was obtained from liquid-liquid extraction of fermentation media and ethyl acetate solvent. That crude extract was then fractionated using vacuum liquid chromatography (VLC). Thin layer chromatography (TLC) test was performed on crude extract and all fractions with ethyl acetate – n-hexane (7:3) and ethyl acetate – methanol (3:1) as eluents. Fractionation of crude extract from vacuum liquid chromatography produced 15 fractions with different spot patterns seen under 254 nm UV lamp. Keywords: antimicrobial, co-culture, secondary metabolites
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    FRAKSINASI KROMATOGRAFI KOLOM METABOLIT SEKUNDER DARI Penicillium sp. LBKURCC34 BERPOTENSI ANTIBAKTERI Escherichia coli
    (2021-03) Sary, Dian Novita; Hendra, Rudi; Nurulita, Yuana
    Penicillium sp. LBKURCC34 is a a producer of secondary metabolite compounds that has several biological activities such as antibacteria and is known as of antibiotics. In this study, Penicillium sp. LBKURCC34 was tested as a producer of antibacterial active compounds against E. coli. Ethyl acetate crude extract from 14 days fermentation of Penicillium sp. LBKURCC34 was tested by TLC by several ration of eluants, such as ethyl acetate: n-hexane (1:1; 3:2; and 7:3) with UV light (254 and 366 nm). Column chromatography separation produced 13 fractions with some of the fractions have antie. coli.
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    IDENTIFIKASI GEN tet BAKTERI TANAH GAMBUT HUTAN ALAMI CAGAR BIOSFER GIAM SIAK KECIL BUKIT BATU DIISOLASI DENGAN MEDIA ANTIBIOTIK
    (perpustakaan UR, 2021-06) Amalia, Aisyah Sabrina; Nurulita, Yuana
    Bacterial isolates named LBKURCC419, LBKURCC423 and LBKURCC424 are natural forest soil bacteria isolated using tetracycline and amoxicillin media from the Giam Siak Kecil Bukit Batu Biosphere Reserve, Riau Province. In this research, these bacteria were assessed by molecular biological techniques to know whether the isolates contain antibiotic resistance genes especially tet (tetracycline). The bacterial chromosomal DNA was isolated using the TIANamp DNA bacteria kit, then continued to determine the genes encoding tetracycline resistance using polymerase chain reaction (PCR) with eight primers of the tet genes according to the respective annealing temperature. PCR products were identified by electrophoresis. The results showed the eight tet genes were identified negative that meant the isolates did not have that gene. This can occur because the tested resistant genes may be present in plasmids while in this study perhaps the isolated DNA came from chromosomes so that it did not contain the resistant genes tested. In addition, the nature of low resistance to bacteria because it comes from natural soil also causes the mechanism of bacterial resistance to be unknown.
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    PENGARUH BERBAGAI pH TERHADAP SPEKTRUM ZAT WARNA TEKSTIL REACTIVE BLACK 5
    (Elfitra, 2023-01) Nabilah, Putri; Nugroho, Titania Tjandrawati; Nurulita, Yuana
    Reactive Black 5 (RB5) dyes are a group of azo dyes that are widely used for dyeing fabrics. The purpose of this study was to determine the effect of the pH used in different variations, namely at pH 4,5, pH 5,5 and pH 6,5 on the resulting RB5 color spectrum, as measured by its absorbance of visible light. Based on this study, there were no significant differences observed in the visible light absorbance maximum wavelengths of RB5 at different pHs. The RB5 visible light absorbance spectrum resulted in a broad peak, with an approximate absorbance maximum at 600 nm at pH 4,5, pH 5,5 and pH 6.5. Although not causing any shift in the maximum absorbance wavelength, there was a difference in the resulting absorbance intensity at different pH. So, it can be concluded that the use of pH variations between pH 4,5 to pH 6,5 can cause differences in absorbance intensity, but no shift in RB5 maximum absorbance wavelength.
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    POLA TIC (Total Ion Current) LC-MS/MS EKSTRAK ETIL ASETAT METABOLIT SEKUNDER DARI MEDIA PERTUMBUHAN Penicillium sp. LBKURCC34 DENGAN VARIASI WAKTU INKUBASI
    (2020-08) Hardianti, Vinkha; Nurulita, Yuana; Haryani, Yuli
    Liquid Chromatography Mass Spectrometry (LC-MS / MS) is an analytical technique that combines the physical separation capabilities of liquid chromatography with the specificity of mass spectrometry detection. This method can identify the differences of compound from the extracts. The ethyl acetate extracts produced from the co-culture of Penicillium sp. LBKURCC34 and Staphylococcus aureus with various incubation times (8 days, 11 days and 14 days) were identified with LC-MS. The results of secondary metabolites obtained showed that the peak of the dominant compound in extracts generally was at retention times ranging from 9-13 min. The data showed that the compound characteristics are more polar. Interestingly, only extract from 14 days incubation contained less polarity compound.
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    POLA TIC (Total Ion Current) LCMS-MS EKSTRAK ETILASETAT DARI PRODUKSI METABOLIT SEKUNDER Penicillium sp. LBKURCC34 DENGAN VARIASI KONSENTRASI GLUKOSA
    (2020-04) Afriani, Anela Putri; Nurulita, Yuana
    Liquid chromatography - mass spectrometry (LCMS) is an analytical chemistry technique that combines the physical separation capabilities of liquid chromatography with the mass analysis capabilities of mass spectrometryis. This tool could identify the differences of extract contains. The differences of secondary metabolites that are produced by co-culture of Penicillium sp. LBKURCC34 and Staphylococcus aureus with variation of glucose concentration (5g/L (Extract A), 10g/L (Extract B) and 15 g/L(Extract C)) were identified by LCMS. This study analyzed the difference patterns of Total Ion Current (TIC) of LCMS chromatogram profiles from these extracts. Based on this research, it was known that three extracts have different pattern of LCMS chromatogram with mostly of the peaks showed polar substances with molecular weight around 84,96 - 655,37 m/z.
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    Produksi Kitinase Trichoclerma Asperellum Tnc52 Dan Tnj63 Galur Lokal Riau Menggunakan Limbah Kulit Udang
    (2015-07-08) Nurulita, Yuana; T. Nugroho, Titania; Devi, Silvera
    Kitin merupakan polimer kedua terbanyak di alam setelah selulosa serta merupakan komponen penyusun tubuli serangga, udang, kepiting, ciimi-cumi, dan artropoda lainnya, serta bagian dari dinding sel kebanyakan fungi dan alga. Setiap tahun dari perairan (laut) dihasilkan sekitar lO" ton kitin, namun kurang dari 0,1% yang dimanfaatkan kembali. Proses degradasi kitin dapat menggunakan biokatalisator yaitu enzim yang tentunya lebih murah, aman, dan cepat jika dibandingkan dengan proses kimiawi tanpa enzim. Proses degradasi kitin menggunakan kitinase. Genus jamur Trichoderma sp. merupakan fungi yang mampu menghasilkan enzim kitinase (Loritto et al., 1993). Meneliti spesies galur ini adalah penting terutama galur lokal Riau T. asperelliim TNC52 dan TNJ63 dari segala aspek pemanfaatannya dalam menghasilkan kitinase. Penelitian ini bertujuan untuk menguji limbah cangkang udang laut dan sungai juga kitin IPB sebagai pembanding dalam memproduksi kitinase dari jamur T. asperellum lokal Riau dengan berbagai konsentrasi substrat yaitu 0,2; 0,5; 1; dan 2,5%. Kitinase yang dihasilkan diuji aktivitasnya dalam mendegradasi kitin koloidal SIGMA dan gula pereduksi yang dihasilkan dimonitor dengan nelson somogyi. Protein kitinase yang dihasilkan ditentukan kadar proteinnya untuk menentukan aktivitas spesifik kitinase.
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    Sintesis Dan Uji Bioaktivitas Beberapa turunan Kurkumin
    (2015-07-05) Eryanti, Yum; Yuharmen; Nurulita, Yuana
    Senyawa kurkumin baik sintetik maupun alami dikenal mempunyai aktivitas biologis yang bervariasi, seperti antioksidan, antibakteri, antiinflamasi, antimalaria dan antikanker. Aktivitas biologis dari senyawa kurkumin berhubungan dengan adanya keton a,p tak jenuh dan kekuatannya dipengaruhi oleh jenis dan pola substituen yang ada pada cincin aromatiknya. Pada laporan penelitian ini dilaporkan dua belas senyawa kurkumin telah berhasi! disintesis dan enam dari senyawa tersebut sudah dilakukan karakterisasinya. Senyawa analog kurkumin dibuat dengan cara merefluks turunan keton (asetofenon, siklopentanon, sikloheksanon), turunan aldehid aromatik (benzaldehid, sinamaldehid, 4- hidroksibenzaldehid,4-aminabenzaldehid) dan sodium hidroksida pelet dalam lumpang. Secara umum rendemen yang dihasilkan cukup tinggi (63-99%), ada tiga senyawa yang mempunyai hasil rendemen dibawah 75% yaitu senyawa yang mempunyai gugus pendorong elektron dalam hal ini adalah amin pada posisi para dari senyawa keton aromatik. Enam senyawa sudah dikarakterisasi dengan metoda spektroskopi UV, IR dan metoda NMR.
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    Sintesis Dan Uji Bioaktivitas Beberapa Turunan Kurkumin
    (2015-07-05) Eryanti, Yum; Yuharmen; Nurulita, Yuana
    Senyawa kurkumin baik sintetik maupun alami dikenal mempunyai aktivitas biologis yang bervariasi, seperti antioksidah, antibakteri, antiinflamasi, antimalaria dan antikanker. Aktivitas biologis dari senyawa kurkumin berliubungan dengan adanya keton a,P tak jenuh dan kekuatannya dipengaruhi oleh jenis dan pola substituen yang ada pada cincin aromatiknya. Pada laporan penelitian ini dilaporkan dua belas senyawa kurkumin telah berhasil disintesis dan semua senyawa tersebut sudah dilakukan karakterisasinya. Senyawa analog kurkumin dibuat dengan cara merefluks turunan keton (asetofenon, siklopentanon, sikloheksanon), turunan aldehid aromatik (benzaldehid, sinamaldehid, 4- hidroksibenzaldehid, 4-aminabenzaldehid) dan sodium hidroksida pelet dalam lumpang. Secara umum rendemen yang dihasilkan cukup tinggi (63-99%), ada tiga senyawa yang mempunyai hasil rendemen dibawah 75%) yaitu senyawa yang mempunyai gugus pendorong elektron dalam hal ini adalah amin pada posisi para dari senyawa keton aromatik. Semua senyawa sudah dikarakterisasi dengan metoda spektroskopi UV, IR dan metoda NMR dan sepuluh senyawa sudah dilakukan uji aktivitas antioksidan, toksisitas dan antiinflamasi.
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    SITOTOKSISITAS DARI BEBERAPA SENYAWA TURUNAN KURKUMIN PADA SEL P388
    (2014-05-21) Eryanti, Yum; Nurulita, Yuana; Yuharmen; Hendra, Rudi; Zamri, Adel
    Four analogue curcumins (3b, 3e, 3h and 3i) have been synthesized from aldehyde and ketone by using Claisen-Schmidt condensation under base condition. All the compounds have been confirmed their structure by using UN, IR, 1HNMR and 13CNMR spectroscopy analysis. Generally, all the compounds showed cytotoxicity activity with IC50 value 15 – 80 ppm against murine leukemia (P-388) cell lines. The results showed that 3i could inhibit the highest P-388 cell line growth percentage.
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    STUDI ANTAGONISTIK Trichoderma asperellum LBKURCC1 DAN Penicillium sp. LBKURCC34
    (Elfitra, 2023-12) Nasution, Ariq Fadhil Shidiqi; Nurulita, Yuana; Rafi, Mohamad
    Trichoderma asperellum LBKURCC1 and Penicillium sp. LBKURCC34 are a fungi isolated from plantation soil and peat soil in Riau Province. This study aims to examine the antagonistic activity between local isolates of Riau fungi; LBKURCC1 and LBKURCC34. The aim of the antagonistic test is to measure and determine the ability of antagonistic fungi to suppress the growth and development of pathogenic fungi or fungi tested in close proximity to the laboratory scale co-culture method. Antagonistic tests can be carried out in vitro using the dual culture method. Antagonistic tests are carried out for 14 days and observations are made in control and test petris. In the antagonistic test, the LBKURCC1 fungus showed stronger antagonistic inhibition than the LBKURCC34 fungus.
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    UJI AKTIVITAS ANTIMIKROBA EKSTRAK KO-KULTUR ISOLAT JAMUR LOKAL RIAU Penicillium sp. LBKURCC34 DAN LBKURCC38
    (Elfitra, 2023-05) Azzahroh, Vira; Nurulita, Yuana
    Penicillium sp. LBKURCC34 and LBKURCC38 are fungi isolated from peat soil of the primary forest of the Giam Siak Kecil Bukit Batu Biosphere Reserve (GSKBB) of Riau Province. This fungus is known to have the potential to produce secondary metabolites that have antimicrobial activity. This research was conducted using co-culture technique between Penicillium sp. LBKURCC34 with Penicillium sp. LBKURCC38. Extraction was carried out using ethyl acetate (EtOAc), butanol (BuOH) and acetone (Ase) solvents. These extracts were tested for their antimicrobial activity using the disc diffusion method. The results of the antimicrobial activity test showed that the EtOAc extract at a concentration of 2000 μg/disc had antimicrobial activity against four pathogenic microbes namely S. epidermidis, B. subtilis, E. coli, and C. albicans, BuOH extract only had antimicrobial activity against 2 pathogenic microbes namely B. subtilis and C. albicans, whereas at a concentration of 200 μg/disc, the EtOAc extract only had antimicrobial activity against pathogenic microbes S. aureus and C. albicans. BuOH extract only has activity against S. aureus bacteria, while Ase extract only has activity against C. albicans fungus.
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    Uji Antagonistik Dan Antijamur Media Produksi Kitinase Trichoderma asperellum TNJ63 Galur Lokal Riau Pada Beberapa Jamur Patogen Tanaman
    (2015-07-02) Dahliaty, Andi; Nurulita, Yuana; T. Nugroho, Titania
    Trichoderma asperellum TNJ63 adalah galur lokal Riau penghasil kitinase. Kitinase erat kaitannya dengan kemampuan melindungi tanaman terhadap beberapa penyakit tanaman karena kitinase adalah enzim yang mampu memecah kitin yang pada umumnya merupakan komponen diding sel jamur pathogen tanaman. Penelitian yang menguji aktivitas antagonistik dan antijamur dari media produksi kitinase terhadap beberapa jamur pathogen {Fusarium sp, Ganoderma boninens, dan Rhizoctonia solani) perlu dilakukan, sehingga dapat dijadikan studi pustaka pengembangan metode penanggulangan penyakit tanaman di Riau. Uji antagonistik dilakukan dengan dengan cara meletakan potongan jamur pathogen dan jamur uji di atas media agar yang berjarak 2cm, penghambatan diamati setelah 4, 5 dan 6 hari, sedangkan uji antijamur dengan metoda Fungal Growth Inhibition assay yaitu dengan cara meletakan potongan jamur pathogen diatas media yang mengandung enzim kitinase I x dan 2x kuat. Untuk mendapatkan enzim Ix dan 2x kuat enzim dipekatkan dengan menggunakan ultrafiltrasi.
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    UJI ANTIMIKROBA MEDIA FERMENTASI BATCH Penicillium sp. LBKURCC34
    (wahyu sari yeni, 2019-01-31) Fitri, Annisa; Eryanti, Yum; Nurulita, Yuana
    Penicillium sp. LBKURCC34 is a fungi that isolated from peat soil of primary forest at Giam Siak Kecil Bukit Batu (GSKBB), Biosphere Reserve in Riau Province. The purpose of this study was to determine potency of this fungi as the source of antimicrobial compounds toward Escherichia coli, Bacillus subtilis, Staphylococcus aureus, Staphylococcus epidermidis and Candida albicans with dilution method. The metabolite compound production was done by fermentation in liquid medium for 14 days. Extract was evaporated and concentrated, then dissolved with dimethyl sulfoxide. Antimicrobial tests were carried out using resazurin dilution method using extract concentration of 4000 μl diluted to 7.8125 μl with positive controls Amoxsan® and Ketokonazol®. Unfortunately, due to inconsistency of resazurin reaction, the activity could not be calculated.