Browsing by Author "Putri, Nur Aisyah"

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    OPTIMASI SUHU ANNEALING UNTUK AMPLIFIKASI DAERAH atpB-rbcL INTERGENIC SPACER PADA TUMBUHAN DURIK-DURIK (Syzygiumsp.) ASAL RIAU
    (2020-08) Putri, Nur Aisyah; Herman, Herman
    Durik-durik (Syzygium sp.) is a plant from the family Myrtaceae that can grow in the flooded swamp ecosystem. This plant has a good adaptability to inundation stress. This study aimed to determine the optimal annealing temperature that could be used for amplification in the atpB-rbcL intergenic spacer region. Leaf samples of durik-durik (Syzygium sp.) plant were taken from Lake Kajuik located in Langgam Village, Pelalawan Regency, Riau Province. Samples were extracted using Genomic DNA mini kit Plant (Geneaid) then amplified using dreamTaq DNA polymerase and atpB-rbcL intergenic spacer primer pair with a PCR machine. The results showed that DNA bands were around 1000 bp. The optimal annealing temperature was Tm-5 = 46,6 oC.