Browsing by Author "Suhyana, Jajang"

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    EKSPLORATION OF GIAM SIAK KECIL-BUKIT BATU BIOSFEER RESERVE FOR CARBOLYTIC FUNGI
    (2014-05-21) Nugroho, TitaniaTjandrawati; Zul, Delita; Martina, Atria; Puspita, Fifi; Sepryani, Harni; Ismet, Rhahma Sari; Suhyana, Jajang; Utamy, WidyaRahmy
    GiamSiak Kecil-Bukit Batu (GSKBB) Biosphere Reserve in Riau Province, Indonesia, has a unique tropical peat swamp forest with peat depth reaching up to 20 meters deep. As a tropical peat swamp forest it is a potential source for carbolytic enzyme producing microbes. In this paper we present our exploration at two different sites of the GSKBB Biosphere Reserve forest, representing primary and secondary forest, for carbolytic fungi. Although the isolation media was design for the specific isolation of Trichoderma, we isolated carbolytic enzyme producers from the genus Trichoderma, PenicilliumandAspergillus. From the primary forest, a total of four cellulase and two chitinase producing fungi were isolated to purity. From the secondary forest, a total of two cellulase and one chitinase producing fungi were isolated to purity. Highest specific activity of cellulase was produced by a Penicillium secondary forest isolate. Highest specific activity of chitinase was produced by an Aspergillus primary forest isolate, with a total chitinase crude extract specific activity of 0.04 U/mg at 40oC, pH 5.5.
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    Isolasi Dan Penentuan Aktivitas Enzim Kitinase Trichoderma Rhizosphere Hutan Gambut Giam Siak Kecil-Bukit Batu Riau
    (2015-07-04) Suhyana, Jajang
    Chitinolytic fungi were isolated from soil of natural forest Giam Siak Kecil-Bukit Batu, Riau. That isolate was namely LBKURCC17, have identified as Trichoderma sp. That isolate was cultured in the selective medium containing chitin source for 9 days to produce chitinase. It was not observed that activity of chitinase because the researh only identified specific fungi that have chitinase activity.