Browsing by Author "Zul, Delita"

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    AKTIVITAS FOSFATASE TANAH DAN TOTAL POPULASI BAKTERI PELARUT FOSFAT: DAMPAK ALIH FUNGSI LAHAN GAMBUT MENJADI PERKEBUNAN SAGU DI RIAU
    (2017-01-03) Handayani, Fitri; Zul, Delita; Fibriarti, Bernadeta Leni
    Sebagian besar lahan gambut di Riau telah diubah untuk kegiatan pertanian. Konversi lahan tersebut mengakibatkan perubahan jenis vegetasi yang mengganggu siklus P dalam tanah karena adanya perubahan populasi dan aktivitas mikroba. Tujuan penelitian ini adalah untuk menganalisis dampak konversi lahan gambut menjadi perkebunan sagu terhadap karakteristik fisika-kimia tanah, aktivitas fosfatase tanah, dan total populasi bakteri pelarut fosfat. Sampel tanah diambil dari tujuh lokasi yang berbeda di Desa Lukun, Kecamatan Tebingtinggi Timur, Kabupaten Meranti, Riau, yaitu hutan alam (HA), perkebunan sagu yang ditanam dengan umur 6 bulan (HSB), usia tanaman sagu 7 tahun yang bercampur dengan karet umur 5 tahun (HS+K), usia perkebunan sagu 20 tahun (HS 20 TH), usia perkebunan sagu 40 tahun (HS 40 TH), usia perkebunan sagu 80 tahun (HS 80 TH), dan usia perkebunan sagu 120 tahun (HS 120 TH). Karakter fisika-kimia tanah diukur dengan mengikuti metode standar. Aktivitas fosfatase tanah diukur dengan menggunakan metode kolorimetri dan total populasi bakteri pelarut fosfat ditentukan dengan menggunakan metode Total Plate Count. Karakter fisika-kimia tanah bervariasi dengan kisaran pH 5,63-6,50, suhu 28,25-31,25°C, kelembaban 58,75-86,25%, berat kering tanah 0,68-0,79 g, kandungan air tanah 21,00-32,00%, berat volume tanah 0,21 -0,27 g/cm3. Aktivitas fosfatase asam tertinggi ditemukan di HS 40 tahun (40,33 mol PNP/hr/g tanah) dan terendah di HSB (13,71 mol PNP/hr/g tanah). Total bakteri pelarut fosfat tertinggi pada HS 40 tahun (1,68×104 CFU/g tanah) dan terendah pada HSB (0,12×104 CFU/g tanah). Berdasarkan data, dapat disimpulkan bahwa konversi lahan gambut menjadi perkebunan sagu mempengaruhi aktivitas fosfatase asam, tetapi tidak mempengaruhi total populasi bakteri pelarut fosfat.
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    Aktivitas Ligninolitik Jamur Aphylloporales Strain Local Penghasil Lignin-Modifying Enzymes
    (wahyu sari yeni, 2017-09-07) Martina, Atria; Zul, Delita
    Jamur yang mempunyai aktivitas ligninolitik berpotensi diterapkan pada bidang bioteknologi seperti pada proses biopulping, biobleaching, dekolorisasi dan detoksifikasi limbah-limbah pabrik yang bersifat toksik dan sangat sulit terurai di lingkungan. Aktivitas ligninolitik dihasilkan oleh lignin-modifying enzymes yang terdiri dari lignin peroksidase, mangan peroksidase, dan lakase. Penelitian tentang aktivitas ligninolitik terhadap 14 jenis Aphyllophorales strain lokal yang berasal dari TNBT menggunakan prosedur lempeng agar pada medium agar “N-Limited-RBB” yang mengandung lignoselulosa dan lignin sintesis yaitu indulin AT. Seleksi isolat yang mempunyai aktivitas ligninolitik berdasarkan pembentuka zona perubahan warna pada medium selama inkubasi 6 hari pada suhu 37oC. Hasil penelitian menunjukan bahwa isolat yang mempunyai aktivitas ligninolitik pada medium mengandung indulin adalah Ganoderma sp, Stereum sp, dan AMS 27. Pada medium yang mengandung lignoselulosa, isolat yang mempunyai aktivitas ligninolitik adalah Ganoderma sp, Strereum sp, AMS 27, Polyporus sp2, Microporus sp1, dan Formes sp. Ganoderma sp merupakan isolat yang mempunyai aktivitas ligninolitik tertinggi pada kedua tipe lignin dengan diameter zona perubahan warna pada indulin AT adalah 8,71 cm dan pada lignoselulosa adala 9,71 cm.
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    BAKTERI PENGHASIL BIOPLASTIK DARI LIMBAH INDUSTRI SAGU DAN TAHU DI RIAU: ISOLASI, SELEKSI, DAN OPTIMASI PRODUKSI
    (perpustakaan UR, 2021-07) Febri, M; Zul, Delita
    Conventional plastic is difficult to degrade and can pollute the environment. Therefore, an alternative to conventional plastic is needed by using bioplastics that are more easily biodegradable. The purpose of this study was to obtain potential bacterial isolates to produce PHA as raw material for bioplastics from industrial waste of sago and tofu in Riau. PHA-producing bacteria were isolated using Nutrient Agar medium using the spread plate method. Qualitatively, PHAproducing bacteria were selected based on their ability to absorb the Sudan black solution. The selected bacterial isolates were cultured in mineral salt medium (MSM) for the production of PHA and the resulting PHA was extracted using chloroform. A total of 5 isolates that showed high color absorption intensity were isolated from sago factory waste located in Goguk Village, Meranti Islands. The highest PHA accumulation was produced in 50% palm oil mill liquid waste (LCPKS) medium by LPS1_9 isolate, while the lowest PHA accumulation was produced in MSM medium by LPS1_12 isolate. The use of 50% LCPKS as a carbon source tends to support an increase in the accumulation of PHA, compared to the use of a carbon source from a minimal nutrient medium MSM.
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    BAKTERI PENGHASIL BIOPLASTIK DARI TANAH GAMBUT RIAU: ISOLASI SELEKSI DAN OPTIMASI PRODUKSI
    (perpustakaan UR, 2021-07) Marliana, Anggi; Zul, Delita
    Synthetic plastic has the disadvantage of being difficult to decompose in nature, causing environmental problems. To overcome the problem of accumulation of plastic waste, the use of environmentally friendly plastics (bioplastics) is one solution. Bioplastics are made from natural polymers such as starch, cellulose, and fats. Polyhydroxyalkanoate (PHA) is one of the building blocks of bioplastics. PHA is the result of microbial metabolism produced by bacteria as a response to an unbalanced environment. Bacteria that have the ability to produce PHA can be isolated from various sources such as industrial waste, agricultural waste and soil. This study aims to isolate PHA-producing bacteria from peat soil, Bengkalis, Riau and optimize PHA production through modification of production media. Peat soil samples were taken from six types of locations with four replications, namely, industrial plantation forest, restoration land, rubber plantation, secondary forest, oil palm plantation and burnt land. Isolation was carried out using the spread plate method and qualitative selection of PHA-producing bacteria was carried out using suddan black dye. A total of 10 bacterial isolates had a high ability to produce PHA which was indicated by the black color absorption. The highest accumulation of PHA was 46.47% of HS4_8 isolates using 50% LCPKS enriched medium.
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    Biodegradasi Herbisida Atrazin Oleh Rhizobium Spp Indigenus Diisolasi Dari Nodul Akar
    (wahyu sari yeni, 2017-09-13) Martina, Atria; Zul, Delita
    Atrazin merupakan herbisida yang sering digunakan pada pertanian dan sering mengkontaminasi tanah dan air tanah akibat intensifikasi pertanian yang tidak terkontrol. Atrazin sangat toksik dan sukar terdegradasi dalam tanah. Beberapa jenis mikroba tanah mampu mendegradasi herbisida ini. Bakteri rhizobium yang hidup bebas di tanah dan daerah perakaran tanaman legum dapat mendegradasi atrazin. Penelitian ini bertujuan untuk memperoleh isolat Rhizobium spp serta menguji kemampuannya dalam mendegradasi herbisida atrazin. Rhizobium spp diisolasi dari nodul akar tanaman legum. Sampel diambil secara acak di tiga desa di Kec. Kampar. Masing-masing daerah dilakukan 5 pencuplikan dengan 3 kali pengulangan. Seleksi dilakukan 2 tahap. Tahap pertama menggunakan garam mineral yang mengandung atrazin 5 mg/l dengan waktu inkubasi satu minggu sedangkan tahap kedua dengan konsentrasi atrazin 10 mg/l diinkubasi 8 hari. Hasil penelitian menunjukkan pada konesentrasi atrazin 5 mg/l terdapat 22 isolat Rhizobium yang mampu tumbuh. Pada konsentrasi atrazin 10 mg/l terdapat 9 isolat yang membentuk zona perubahan warna dan berarti mampu mendegradasi atrazin. Rhizobium sp isolat RA 4.2 merupakan isolat yang mempunyai kemampuan paling cepat dan tertinggi dengan diameter zona perubahan warna 9,98 cm. Lima isolat mempunyai kemampuan degradasi terendah dan pembentukan zona perubahan warna yang lambat dengan diameter zona 0,6 cm
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    Biomasa Karbon Mikroba dan Aktivitas Enzim Selulolitik di Cagar Biosfer Giam Siak Kecil-Bukit Batu: Status Hara Karbon
    (2012-10-25) Yunita, Melda; Zul, Delita; Fibriati, Bernadeta Leni
    Giam Siak Kecil-Bukit Batu Biosphere Reserve is a peatland ecosystem consisting higher biodiversity of flora, fauna and soil microbes. Most of this areas have been converted into industrial forest plantation, oil palm plantation, agricultural activities, and settlement. This activity will decrease vegetation number and influence microbial population and activities at the end. Changing of the microbial at those population and activities will affect rate the impact of C cycle in peatland. This research aim was to analyze the impact of land use changes to microbial carbon biomass, cellulolytic bacterial cells number, and rate of cellulolytic enzymes activity (celllulase and β-glucosidase). Soil samples were taken from six different locations in Giam Siak Kecil-Bukit Batu Biosphere Reserve, namely primary forest (as a control), secondary forest, fourteen years old rubber plantation, fourty to sixty years old rubber plantation, twelve years old oil palm plantation, and three years old oil palm plantation. Soil physical and chemical characteristics varied in every location ranging from 28.25-31.25 ( o C) for soil temperature, dry weight 14.35-33.10 (%); water content 72.07-83.30 (%); bulk density 0.06-0.33 (g/cm 1 3 ) and 3.5 for soil pH. The lowest number of microbial carbon biomass was shown in the twelve years old oil palm plantation (240.29 µg C/hour/g soil) and the highest was in the primary forest (963.95 µg C/hour/g soil). The lowest number of cellulolytic bacterial cells number was shown in the primary forest (2.4·10 3 CFU/g soil) and the highest was in the fourty to sixty years old rubber plantation (1.3·10 4 CFU/g soil). The lowest number of cellulase activity was shown in the primary forest (0.67 µg/hour/g soil) and the highest was in the fourty to sixty years old rubber plantation (4.17 µg/jam/g tanah). The lowest number of β-glucosidase activity was shown in the 3 years old oil palm plantation (5.92 µmol PNP/hour/g soil) and the highest was in the fourty to sixty years old rubber plantation (27.45 µmol PNP/hour/g soil). Generally, land use changes govern the biomass microbial carbon biomass, cellulolytic bacterial number, and rate of cellulolytic enzymes activity in Giam Siak Kecil-Bukit Batu Biosphere Reserve.
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    BIOMASSA FOSFOR MIKROBA DAN FOSFATASE TANAH GAMBUT DI SEMENANJUNG KAMPAR: ANALISIS DAMPAK ALIH FUNGSI LAHAN TERHADAP STATUS UNSUR FOSFOR
    (2014-03-28) Rahmila; Zul, Delita; Fibriarti, Bernadeta Leni
    Kampar Peninsula is one of the peat areas that has a high conservation value in Riau. Most of this areas have been converted into agriculture, plantations, and industrial plantation forests. This activity will reduce the amount of vegetation and affect microbial community composition and activity, which in turn will affect the P cycle in peatlands. This study aims to analyze the impact of land use to microbial P biomass and soil acid phosphatase activity. Soil samples were taken from three different land use systems, namely secondary forest, the 7-8 year old palm plantation and cassava fields. Microbial P biomass was quantified by using Chloroform Fumigation Extraction (CFE) method, while soil acid phosphatase activity was determined by using of colorimetric method. Microbial P biomass was high in the secondary forest site (274,27 μg P/g soil), and the lowest was in the cassava fields (72,68 μg P/g soil). Soil acid phosphatase was very active in the secondary forest site (40,58 μmolPNP/hours/g soil) and the lowest activity was found in the cassava fields (22,68 μmolPNP/hours/g soil). From the data, it can be concluded that land use affects the microbial P biomass and acid phosphatase activity in The Kampar Peninsula
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    Biomassa Nitrogen (N) Mikroba dan Aktivitas Urease Tanah di Cagar Biosfer Giam Siak Kecil-Bukit Batu: Sebagai Status Hara Nitrogen
    (2013-03-04) Komariah, Siti; F, Bernadeta Leni; Zul, Delita
    Giam Siak Kecil-Bukit Batu Biosphere Reserve (GSK-BB) is one of the peatland ecosystem that most of the regions have been converted into oil palm plantations, industrial forest plantation, and settlement. Environmental conditions disrupted by land conversion will affect the population, diversity and activity of soil microbes. This research aim was to analyze the impact of land use changes to nitrogen fixation bacterial cells number, microbial nitrogen biomass and urease activity in peatlands. Nitrogen fixation bacterial cells number was calculated by using a Total Plate Count (TPC) method, concentration of microbial N biomass was determined by using Chloroform Fumigation Extraction (CFE) and rate of urease activity was determined by quantifying ammonia released during an incubation by adding buffer solution. The result of physical and chemical characteristic of the soil ranged from 3.5 for pH, temperature 28.25-31.25oC, dry weight 21.20-42.86%, water content 63.2- 78.8% and bulk density 0.10-0.34 g/cm3. Cells number of nitrogen fixation symbiotic bacteria ranged from 3⋅2-4⋅9⋅103 CFU/g soil and cells number of nitrogen fixation non- symbiotic bacteria ranged from 3⋅2-9⋅3⋅102 CFU/g soil. Microbial N biomass varied ranging from 1.15-4.29 μg N/g soil and urease activity varied ranging from 3.66-39.28 μg NH4+/g dry weight soil/h. Based on the results obtained, it can be described that the soil quality in the GSK-BB Biosphere Reserve is still capable enough to support the sustainability of soil biological processes
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    DETEKSI BACTERIAL KEY SPECIES PADA HUTAN SEKUNDER DAN LAHAN BEKAS TERBAKAR: SEBAGAI UPAYA PENENTUAN BARKODE DNA
    (2021-05) Zebua, Putri Kristina; Zul, Delita
    Peatlands are the result of the decomposition of organic matter under anaerobic conditions that naturally accumulated over hundreds of years. A massive conversion of land function can lead to peatland degradation which has an impact on the degradation of the peatland quality. Currently, more sensitive indicators are needed to detect the initial disturbance of peatland degradation. This study aimed to determine the bacterial key species (BKS) in secondary forest areas and burnt areas which can then be used as DNA barcodes to monitor the quality of peatlands. DNA barcodes were obtained by determining BKS with the criteria of finding BKS that were only found in secondary forest and not found in locations that had been converted. This study used 16S rRNA gene sequence data generated from sequencing with the next generation sequencing (NGS) method. This research method included processing FastQ into fasta format using the Galaxy program, construction of phylogenetic trees using the MEGA program version 6.06, determining BKS candidates based on phylogenetic tree construction, selection of BKS candidates using BLASTn analysis: Align Two or More Sequence, alignment and editing of candidate DNAbarcode determination, primer design and in silico PCR analysis using the FastPCR application. The results of this study showed that 3 BKS were successfully detected which were only found in secondary forest locations but not found on burnt land (BKS_SLT). There are 2 candidates for the DNA barcode on BKS_SLT, namely the BKS_SLT2 barcode with 6 primers and the BKS_SLT3 barcode with 8 primers. The primers chosen were primers that had been tested for their sensitivity through in silico PCR.
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    EFEKTIVITAS PENGUKURAN HIDROLISIS FLUORESCEIN DIACETATE SEBAGAI INDIKATOR KUALITAS TANAH GAMBUT
    (2021-05) Mustava, M; Zul, Delita
    Giam Siak Kecil-Bukit Batu (GSK-BB) Biosphere Reserve is one of the areas in Riau Province which has the largest area of peatland. Most of the peatlands in Riau have been converted into plantations and industrial plantations (HTI). The conversion of land functions has an impact on changes in vegetation, decreases in the surface of the peat, and affects the quality of the peat soil. This study aimed to determine the effectiveness of fluorescein diacetate (FDA) hydrolysis measurement as an indicator of peat soil quality. In addition, measurements of the physico-chemical characteristics of the soil were also carried out. Peat soil samples were taken from secondary forest (HS), industrial forest plantations (HTI), oil palm plantations (KS), rubber gardens (KK), burnt land (LBT), and restoration land (LR) using a purposive sampling method. Soil physicochemical characters were calculated using standard methods with varying pH results between 3,22-4,22, soil temperature ranged from 27,0-30,25 ℃, soil volume weight ranged from 0,16 to 0,21 g/cm3, soil dry weight ranged from 0,18 to 0,24 g, and groundwater content ranged from 282,02- 442,42 %. The FDA hydrolysis activity ranged from 37,15 to 91,17 μg fluorescein/g soil dry weight/hour. Rubber plantation locations had the highest FDA hydrolysis activity and industrial plantations had the lowest hydrolysis activity. In general, land use change has an effect on changes in the physicochemical character of the soil and the hydrolysis activity of the FDA. From the research results, it can be concluded that the measurement of FDA hydrolysis activity is effective enough to be used as an indicator of the quality of peat soils.
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    EKSPLORASI BAKTERI PROBIOTIK PADA MAKANAN TRADISIONAL PEKASAM DI KABUPATEN KUANTAN SINGINGI
    (2013-02-20) Awaluddin, Amir; Zul, Delita; Pato, Usman
    Penduduk di Negara-negara maju, kni lebih hati-hati untuk menentukan jenis makanan apa yang akan di konsumsinya. Selain cita rasa, merekan juga mulai mempertimbangkan efek dari makanan tersebut untuk kesehatan dalam jangka pendek maupunjangka panjang, serta memikirkan dampak dari proses produksi makanan tersebut terhadap kelestarian lingkungan. Makanan yang berkembang dari kebudayaan suatu daerah pada kurun waktu yang cukup lama disebut dengan makanan tradisional. Indonesia memiliki berbagai jenis makanan tradisional yang sangat potensial di kembangkan dalam meningkatkan gizi masyarakat serta menggali pengetahuan tradisional masyarakat untuk dikembangkan menjadi produk yang memiliki nilai ekonomi lebih tinggi.
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    EKSPLORATION OF GIAM SIAK KECIL-BUKIT BATU BIOSFEER RESERVE FOR CARBOLYTIC FUNGI
    (2014-05-21) Nugroho, TitaniaTjandrawati; Zul, Delita; Martina, Atria; Puspita, Fifi; Sepryani, Harni; Ismet, Rhahma Sari; Suhyana, Jajang; Utamy, WidyaRahmy
    GiamSiak Kecil-Bukit Batu (GSKBB) Biosphere Reserve in Riau Province, Indonesia, has a unique tropical peat swamp forest with peat depth reaching up to 20 meters deep. As a tropical peat swamp forest it is a potential source for carbolytic enzyme producing microbes. In this paper we present our exploration at two different sites of the GSKBB Biosphere Reserve forest, representing primary and secondary forest, for carbolytic fungi. Although the isolation media was design for the specific isolation of Trichoderma, we isolated carbolytic enzyme producers from the genus Trichoderma, PenicilliumandAspergillus. From the primary forest, a total of four cellulase and two chitinase producing fungi were isolated to purity. From the secondary forest, a total of two cellulase and one chitinase producing fungi were isolated to purity. Highest specific activity of cellulase was produced by a Penicillium secondary forest isolate. Highest specific activity of chitinase was produced by an Aspergillus primary forest isolate, with a total chitinase crude extract specific activity of 0.04 U/mg at 40oC, pH 5.5.
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    ENUMERASI TOTAL POPULASI BAKTERI PENAMBAT NITROGEN (N) TANAH GAMBUT DI TELUK MERANTI
    (2014-03-28) Agustine, Metha; Zul, Delita; Fibriarti, Bernadeta Leni
    Teluk Meranti is one of the peatland ecosystems that most of the regions have been converted into oil palm plantations, industrial forest plantation, and settlement. Environmental conditions disrupted by the land conversions will affect the population, diversity, and activity of soil microbes. The objective of the study was to analyze the impact of land use changes to nitrogen fixing bacterial cells number. The nitrogen fixing bacterial cells number was calculated by using a Total Plate Count (TPC) method. The results showed that the physical and chemical characteristics of the soil ranged from 3,63- 4,00 for pH, temperature 27oC-31,5°C, dry weight 14,35-29,70%, water content 70,30- 85,70% and bulk density 0,15-0,39g/cm3. The nitrogen fixing symbiotic bacteria cells number ranged from 3,6-10,0·104 CFU/g soil and the nitrogen fixing nonsymbiotic bacteria cells number ranged from 0,78·10-1,8·104 CFU/g soil. Based on the results obtained, it can be concluded that the soil quality in the Teluk Meranti is still capable enough to support the sustainability of soil biological processes
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    ENUMERASI TOTAL POPULASI MIKROBA TANAH GAMBUT DI TELUK MERANTI KABUPATEN RIAU
    (2014-03-28) Fitria, Rahmi; Zul, Delita; F, Bernadeta Leni
    Teluk Meranti is one of the peatland area in Riau province. Most of these lands have been changed into palm oil plantation, timber plantation, agricultural area and settlement. The aim of this research was to analyze the impact of land use changes on soil physical-chemical characteristics and microbial cell number. Soil samples were taken from eight different locations, namely primary forest as control, secondary forest, rubber plantation (15 monthsyears old), rubber forest (40-60 years old), palm oil plantation (7-8 yearsold), acacia plantation (2-3 years old), corn field, and cassava field. Microbial cell number was determined by spread plate method, employing appropriate media for the growth of bacteria, fungi and actinomycetes. The results showed that the soil humidity, soil temperature, percentage of soil dry weight, water content, soil bulk density and pH ranged from 29,63-55,88%, 27-31,5o C, 14,9-35,5%, 64,9-85,1%, 0,16-0,39g/cm 3 and 3,63-4,00, respectively. The copiotrophic bacterial cell number ranged from 0,6x105-1,8x105 CFU/g soil where the highest population was at the palm oil plantation,whereas the oligotrophic bacterial cell number ranged from 0,5x105-1,4x105 CFU/g soil where the highest population was at the palm oil plantation. The population of fungi ranged from 0,4x105-1,0x105 CFU/g soil where the highest population was at the corn field. The population of actinomycetes ranged from 0,4x105-10,7x105 CFU/g soil where the highest population was at the palm oil plantation. Land use changes caused microbial cell number increased. The results indicated that land use changes influenced the microbial cell numbers
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    FORMULASI BACILLUS SP SEBAGAI ANTIMIKROBA DAN PUPUK ORGANIK
    (2014-06-11) Puspita, Fifi; Restuhadi, Fajar; Zul, Delita
    Penggunaan Bacillus sp dalam mengendalikan penyebab penyakit seperti jamur bercak daun, hawar daun mempunyai beberapa kendala di antaranya adalah tidak dapat bertahan (survival) terutama di filosfer, penanganan penyimpanan dan transportasi. Untuk mengatasi permasalahan ini maka dicari suatu formulasi untuk mempermudah aplikasi dan mempunyai kemampuan patogenisitas yang lama maka formulasi yang akan digunakan adalah formulasi dalam bentuk tepung (powder) berbahan aktif Bacillus sp. Bacillus sp jika dikombinasikan dengan bahan-bahan amandement diharapkan dapat mengoptimalkan daya kerja Bacilllus sp karena bahan-bahan amandemen seperti limbah sagu, limbah padat kelapa sawit mampu menyediakan nutrisi terhadap Bacillus sp. Aplikasi yang banyak dilakukan dan paling mudah digunakan untuk perbanyakan masal atau formulasi Bacillus sp adalah dalam bentuk tepung. Pembuatan formulasi Bacillus sp (BioBs-L WP) yang meliputi tahapantahapan sebagai berikut: perbanyakan masal Bacillus sp, persiapan bahan-bahan amandemen, produksi masal BioBs- L WP, pembuatan formulasi BioBs- L WP. Hasil uji keempat formulasi Bacillus sp sebagai antimikroba mampu menghambat pertumbuhan X. oryzae pv oryzae secara in vitro. Hasil penelitian diperoleh bahwa formulasi BS-TSG dan BS-TK mengandung unsur hara makro N 1.65 % dan 1.64%. dan P dan K tertinggi terdapat pada formulasi BS-LPS yaitu 2.17 % dan 3.67%. Unsur hara Ca (ppm) yang tertinggi terdapat pada formulasi Bs-TKU yaitu 29.9 ppm(tinggi), Mg tertinggi terdapat pada formulasi Bs- TKU yaitu 11,28 (tinggi). Kandungan Fe tertinggi yang terdapat dalam formulasi BS- TKU yaitu 298,96 ppm (tinggi) dibandingkan dengan formulasi lainnya. Kandungan Cu dan Zn tertinggi terdapat paa formulasi BS-LPS yaitu 170.10 ppm (tinggi) dan 465.34 ppm.
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    ISOLASI BAKTERI PENGHASIL POLIHIDROKSI ALKANOAT DARI TANAH GAMBUT DAN TANAH TEMPAT PEMBUANGAN AKHIR SAMPAH DI RIAU
    (2021-02) Maria, Santa; Zul, Delita
    Conventional plastics are difficult to degrade and can pollute the environment. Therefore, another alternative to replace conventional plastic is needed by using bioplastics which are more biodegradable. The purpose of this study was to obtain potential bacterial isolates to produce Polihidroksi Alkanoat (PHA) as a bioplastic feedstock from peat soil and landfills in Riau. PHA producing bacteria were isolated using Nutrient Agar medium with the spread method. PHA producing bacteria were selected using Sudan Black dye and indicated with blackish blue. The selected bacterial isolates were cultured on mineral salt medium for the production of PHA and the resulting PHA was extracted using chloroform. A total of 43 from 182 bacterial isolates obtained from four sampling locations were able to produce PHA. The reselection results showed that 16 of the isolates showed consistency in their ability to produce PHA which was indicated by the absorption of sudan black staining by the bacterial colony. Cell dry weight of the selected isolates ranged from 0.03-2.20 g/L, dry weight of PHA ranged from 0.03-0.43 g/L, biomass residues ranged from 0.16 to 2.17 g/L, and PHA accumulation ranged from 1.17% -63%. The highest PHA accumulation was produced by TPAS2.1 isolates obtained from landfills and the lowest by KB2.3 isolates from orchards.
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    ISOLASI BAKTERI PENGHASIL SELULOSA DARI LIMBAH INDUSTRI KARET ASAL RIAU DAN BUAH-BUAHAN TROPIS (MANGGIS DAN KAPULASAN): APLIKASI UNTUK PRODUKSI KERTAS
    (2020-10) Hanifa, Hanifa; Zul, Delita
    The use of cellulose from plants for paper production continuously requires energy and can damage the environment, so an alternative source of renewable cellulose is needed with more efficient results, namely the production of cellulose from bacteria. Therefore, this study aimed to isolate and test cellulose-producing bacteria from mangosteen, durian, kapulasan and industrial rubber waste for paper production applications. The method used was the isolation of cellulose bacteria using the Hestrin Schramm (HS) medium, the selection of selected isolates using the Glucose Extract Yeast (GEY) medium, then A production test was carried out using a modified coconut water medium under static conditions for 14 days and the determination of bacterial cellulose production. The results showed that as many as 6 isolates (MSB-1b, MSB-2b, MSB-5b, PULR-1b, PULR-2b and PULR-2c) that were tested were successful in forming cellulose mats and none were successful in forming cellulose pellicles. The z / k ratio values of each isolate were MSB-1b = 2.15, MSB-2b = 2.70, MSB-5b = 2.85, PULR-1b = 5.93, PULR-2b = 5.30 , and PULR-2c = 5.10. Based on the research results, the z / k ratio cannot be used as a reference in selecting the ability of cellulose bacteria to form pellicles, so it cannot be known about its ability as a material for paper production.
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    ISOLASI BAKTERI PENGHASIL SELULOSA DARI LIMBAH PERKEBUNAN KELAPA SAWIT DAN BUAH-BUAHAN ASAL RIAU (NANAS DAN SAWO) ALTERNATIF PENGGANTI KAPSUL NON-HALAL
    (2020-10) Rahma, Hilyana; Zul, Delita
    Bacterial cellulose is pure cellulose synthesized by various species of bacteria. Bacterial cellulose can be applied in the medical field as a substitute for non-halal gelatine. This study purposed to isolate and produce cellulose-producing bacteria which can be applied as materials for halal capsules. Isolation of cellulose-producing bacteria was carried out on palm oil plantation waste and three different tropical fruit sources (pineapple, and sapodilla) from Riau using Hestrin-Schramm media in static culture condition. The determination of dry weight and water content was carried out to know the quality of the cellulose pellicles. In this study, 10 isolates succeeded in forming a clear zone from pineapple dan sapodilla. The Z/K ratio obtained from AC was 4,46-5,43 cm and MZ was 4,03-4,58 cm. However after the production test was carried out, all of these isolates failed to form cellulose pellicles.
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    ISOLASI BAKTERI PENGHASIL SELULOSA DARI TANAH GAMBUT RIAU, BUAH JERUK DAN BUAH ANGGUR: APLIKASI DALAM INDUSTRI SERAT TEKSTIL
    (2020-10) Silitonga, Asido; Zul, Delita
    Efforts to obtain plant cellulose require energy and can damage the environment, so an alternative source of cellulose that can be renewed with a more efficient result is bacterial cellulose. For this reason, this study aims to obtain bacterial isolates that have the potential to produce cellulose pellicles which can be applied in the textile fiber industry. The sample was grown on hestrin-schramm medium and incubated. The cellulose mat formed was then gradually diluted and inoculated into the glucose ethanol and yeast extract medium. The isolates that produced the clear zone were then purified on glucose ethanol and yeast extract medium to measure the Z/K ratio. Then the production is carried out using a modified production medium with the addition of coconut water. The cellulose pellicle formed was then purified and the dry weight and moisture content were calculated. At the time of isolation, only grape and orange samples could produce cellulose mats, while peat soil samples were unable to form cellulose mats and cellulose pellicles. At the time of selection, 8 isolates were obtained from grapes and 5 isolates from citrus fruits that produced clear zones. However, at the time of production, only A8 isolates from grapes were able to produce cellulose pellicles. The resulting cellulose pellicle is thick, dense and white in color.
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    ISOLASI BAKTERI PENGHASIL SELULOSA DARI TANAH MANGROVE DAN BUAH-BUAHAN TROPIS SEBAGAI BIOMATERIAL UNTUK PRODUKSI KERTAS
    (2020-10) Sinaga, Lukcya; Zul, Delita
    Bacterial cellulose is a biopolymer produced by acetic acid bacteria through oxidative fermentation. Bacterial cellulose is used in various industrial fields, especially paper production due to its excellent physical-chemical properties. This study aimed to isolate potential bacteria producing cellulose from mangrove soil in Riau and tropical fruits (rambutan, matoa, apple) to be used as biomaterials for paper production. Bacterial isolation was carried out on Hestrin Schramm (HS) medium and the production stage was carried out on HS production medium and modified production medium. There were 6 potential bacteria producing cellulose isolates; however, none of the isolate cultures were able to produce cellulose pellicle. The isolate cultures only produced a thin cellulose mats floating on the medium. The isolate cultures producing cellulose mats were obtained from apple 1, rambutan 2, 3, and mangrove B2.