Biocompatibility of Porous Alumina-Hydroxyapatite Microcarriers in Stirred Tank Bioreactor for Cell Culture
No Thumbnail Available
Date
2015-12-01
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
The biocompatibility study on porous alumina-hydroxyapatite (HA) microcarriers has
conducted in a spinner vessel bioreactor using Vero cells. The effect of HA-to-alumina mass
ratios on the cell attachment and growth rate of cell was investigated. When HA content in
samples was increased, the cells number attached on surface samples increased as well. A
good compatibility of the Vero cells to all the porous microcarriers, since the cells were
observed already attached at the surface of microcarriers at hours 8 and 120 of incubation
time. The cell growth rates of microcarrier containing 0.0, 0.3 and 1.0 w/w were 0.015, 0.019
and 0.017 hour-1, respectively. Carbon content on porous alumina bodies without HA addition
was 36.03%, it significantly increased 46.14% when 1.0 w/w HA-to-alumina mass ratio was
added.
Description
Keywords
Biocompatibility, alumina, hydroxyapatite, microcarrier, cell culture