SELEKSI DAN UJI AKTIVITAS SELULASE ISOLAT BAKTERI ENDOFIT LBKURCC 392 s/d 418 DARI AKAR MANGROVE KABUPATEN BENGKALIS
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Date
2022-04
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Elfitra
Abstract
Endophytic bacteria from mangrove roots have the potential to produce cellulase enzymes. In this
study, 27 isolates of endophytic bacteria from mangrove roots were selected from the collection
of the Enzyme, Fermentation and Biomolecular Research Laboratory, Department of Chemistry,
FMIPA UNRI. The selection was carried out on selective solid media of carboxyl methyl cellulose
(CMC) 1%, while the crude extract enzyme activity was determined by the 3,5-dinitrosalicylic
acid (DNS) method. The results of the selection of 27 isolates of endophytic bacteria from the
Magrove roots of Bengkalis Regency on 1% CMC selective solid media obtained 22 live isolates
because they were able to produce the cellulase enzyme and 5 isolates not alive. Statistical analysis
with DNMRT at a significance level of 5% and PCA with the highest activity in the plant species
Ceriops tagal (Perr.) C.B. Rob (LBKURCC 409) is (489 ±137) x 10-4 U/mL in 1st cluster and the
lowest activity was in plant species Bruguiera sp. (LBKURCC 393) with an activity value of (28
±17) x 10-4 U/mL in the 4th cluster.
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Keywords
activity, cellulase, endophytic, mangrove, root
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