ANALISIS FILOGENETIK NIBUNG (Oncosperma tigillarium (Jack) Ridl.) “FLORA MASKOT” RIAU BERDASARKAN SEKUEN GEN rbcL DENGAN METODE MAXIMUM PARSIMONY (MP)
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Date
2021-06
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perpustakaan UR
Abstract
Taxonomic studies using morphological markers on nibung are difficult to do, because
generative characters such as flowers and fruits as important characters are difficult to find in
the out of season period. Thus, an alternative analysis using molecular methods is required.
The study aimed to analyze kinship relationships and to reconstruct phylogenetic trees based
on the character of the rbcL gene. DNA Extraction Procedure used CTAB method with
modification. DNA fragments were sequenced at the First Base Laboratories Malaysia
through PT. Genetika Science Indonesia followed by alignment using Bioedit application and
phylogenetic analysis using Maximum Parsimony (MP) methods in PAUP* application
version 4.0a169. The MP analysis grouped three collections of Oncosperma tigillarium into
monophyletic clads with a bootstrap value of 100%. This grouping shows that the three
collections of Oncosperma tigillarium from Bengkalis Island and Oncosperma tigillarium
from Rupat Island have a close kinship based on rbcL genes
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Keywords
Phylogenetic, Oncosperma tigillarium, Maximum Parsimony method, rbcL