ISOLASI DNA TOTAL BAKTERI Bacillus sp. ENDOFIT KELAPA SAWIT
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Date
2016-10-12
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Abstract
Endophytic bacteria are living and saprophyte bacteria associated with plant tissue
without causing harm to the plant. One of endophytic bacteria is from the genus of
Bacillus sp. Molecular analysis using 16S rRNA genes needs to be done to identify and
determine the species of endophytic bacteria. The purpose of this study was to isolate
DNA of endophytic bacteria originated from palm oil plants by using 16S rRNA gene.
This research was conducted from February to May 2016. Bacteria was isolated from 3
healthy palm plants, existed around oil palm crops which were afflicted by disease of
rotting base on the stem. Of each plant, bacteria were isolated from the stem, roots,
stems and leaf. Research methods included the manufacture of medium Nutrient Agar
(NA) and Nutrient Broth (NB), rejuvenation of the isolates of bacteria, bacterial DNA
isolation and electrophoresis. The whole (12) isolates of endophytic bacteria which
successfully isolated showed that the total DNA retrieved was intact, characterized by a
thick and intact band
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Keywords
Bacillus sp, Endophytic bacteria, Isolation DNA, Gen 16S rRNA, Palm oil